Generating a self-organizing kidney from pluripotent cells

Little, Melissa H. and Takasato, Minoru (2015) Generating a self-organizing kidney from pluripotent cells. Current Opinion in Organ Transplantation, 20 2: 178-186. doi:10.1097/MOT.0000000000000174


Author Little, Melissa H.
Takasato, Minoru
Title Generating a self-organizing kidney from pluripotent cells
Journal name Current Opinion in Organ Transplantation   Check publisher's open access policy
ISSN 1087-2418
1531-7013
Publication date 2015-04
Year available 2015
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1097/MOT.0000000000000174
Volume 20
Issue 2
Start page 178
End page 186
Total pages 9
Place of publication London, United Kingdom
Publisher Lippincott Williams and Wilkins
Collection year 2016
Language eng
Formatted abstract
Purpose of review: Recent studies on the directed differentiation of human pluripotent stem cells report tissue self-organization in vitro such that multiple component cell types arise in concert and arrange with respect to each, thereby recapitulating the morphogenetic events typical for that organ. Such self-organization has generated pituitary, optic cup, liver, brain, intestine, stomach and now kidney. Here, we will describe the cell types present within the self-organizing kidney, how these signal to each other to form a kidney organoid and the potential applications of kidney organoids.

Recent findings: Protocols for the directed differentiation of human pluripotent cells focus on recapitulating the developmental steps required during embryogenesis. In the case of the kidney, this has involved mesodermal differentiation through posterior primitive streak and intermediate mesoderm. Recent studies have observed the simultaneous formation of both ureteric epithelium and nephron progenitors in vitro. These component cell types signal to each other to initiate nephron formation as would occur during development.

Summary: The generation of kidney organoids is a major advance in nephrology. Such organoids may be useful for disease modelling and drug screening. Ultimately, our capacity to generate organoids may extend to the development of tissues for transplantation.
Keyword Directed Differentiation
Human pluripotent stem cell
Kidney
Organoid
Self organization
Modeling Human Development
Tubular Like Cells
Stem cells
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2016 Collection
Institute for Molecular Bioscience - Publications
 
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