Comparison of attenuated and virulent West Nile virus strains in human monocyte-derived dendritic cells as a model of initial human infection

Rawle, Daniel J., Setoh, Yin Xiang, Edmonds, Judith H. and Khromykh, Alexander A. (2015) Comparison of attenuated and virulent West Nile virus strains in human monocyte-derived dendritic cells as a model of initial human infection. Virology Journal, 12 1: 46.1-46.5. doi:10.1186/s12985-015-0279-3


Author Rawle, Daniel J.
Setoh, Yin Xiang
Edmonds, Judith H.
Khromykh, Alexander A.
Title Comparison of attenuated and virulent West Nile virus strains in human monocyte-derived dendritic cells as a model of initial human infection
Journal name Virology Journal   Check publisher's open access policy
ISSN 1743-422X
Publication date 2015-03
Sub-type Article (original research)
DOI 10.1186/s12985-015-0279-3
Open Access Status DOI
Volume 12
Issue 1
Start page 46.1
End page 46.5
Total pages 5
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2016
Language eng
Formatted abstract
Background: The human-pathogenic North American West Nile virus strain (WNVNY99), responsible for the outbreak in New York city in 1999, has caused 41000 infections and 1739 human deaths to date. A new strain of West Nile virus emerged in New South Wales, Australia in 2011 (WNVNSW2011), causing a major encephalitic outbreak in horses with close to 1000 cases and 10-15% mortality. Unexpectedly, no human cases have so far been documented.

Findings: We report here, using human monocyte-derived dendritic cells (MoDCs) as a model of initial WNV infection, that the pathogenic New York 99 WNV strain (WNVNY99) replicated better than WNVNSW2011, indicative of increased viral dissemination and pathogenesis in a natural infection. This was attributed to suppressed viral replication and type I interferon (IFN) response in the early phase of WNVNY99 infection, leading to enhanced viral replication at the later phase of infection. In addition, WNVNY99 induced significantly more pro-inflammatory cytokines in MoDCs compared to WNVNSW2011.

Conclusions: Our results suggest that the observed differences in replication and induction of IFN response between WNVNY99 and WNVNSW2011 in MoDCs may be indicative of their difference in virulence for humans.
Keyword West Nile virus
Dendritic cells
Monocyte
Pathogenesis
Virulence
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 24 Apr 2015, 12:16:38 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences