Placental fibroblast growth factor 21 is not altered in late-onset preeclampsia

Dekker Nitert, Marloes, Scholz-Romero, Katherin, Kubala, Marta H., McIntyre, H. David, Callaway, Leonie K. and Barrett, Helen L. (2015) Placental fibroblast growth factor 21 is not altered in late-onset preeclampsia. Reproductive Biology and Endocrinology, 13 14: . doi:10.1186/s12958-015-0006-3


Author Dekker Nitert, Marloes
Scholz-Romero, Katherin
Kubala, Marta H.
McIntyre, H. David
Callaway, Leonie K.
Barrett, Helen L.
Title Placental fibroblast growth factor 21 is not altered in late-onset preeclampsia
Journal name Reproductive Biology and Endocrinology   Check publisher's open access policy
ISSN 1477-7827
Publication date 2015-03-08
Year available 2015
Sub-type Article (original research)
DOI 10.1186/s12958-015-0006-3
Open Access Status DOI
Volume 13
Issue 14
Total pages 8
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2016
Language eng
Formatted abstract
Background

Preeclampsia (PE) is associated with alterations of placental function. The incidence of PE is higher in insulin resistant states. Women with PE have high circulating levels of the metabolic regulator fibroblast growth factor 21 (FGF21). FGF21 is synthesized in the placenta. The aim of this study was to compare the expression of FGF21, its receptors, downstream targets and transcriptional regulators in placental tissue from pregnancies with and without late-onset PE. Circulating FGF21 in maternal and cord blood was also studied.

Methods


mRNA expression was determined by semi-quantitative real-time PCR and normalized for cellular composition in 17 women with and 20 without PE. Protein expression was quantified by Western Blot. FGF21 levels were measured by ELISA in maternal and cord serum of ten mother-baby dyads per condition.

Results


Placental FGF21 mRNA and protein expression were similar in PE compared with control. Placental mRNA expression of the FGF receptors (1–4) and the co-receptor beta-Klotho was not different between the groups. There was no difference in the expression of the glucose transporters GLUT1, 3 or 4. PPAR-alpha but not PPAR-gamma expression was decreased in PE. Maternal FGF21 serum levels were not significantly different in PE. FGF21 was detected in cord blood of 6 infants (4 PE, 2 controls) but was undetectable in 14 infants.

Conclusions

Late-onset PE is not associated with major changes to the expression of FGF21, its receptors or metabolic targets.
Keyword FGF21
Preeclampsia
Placenta
PPAR-alpha
PPAR-gamma
GLUT
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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