Adult non-cystic fibrosis bronchiectasis is characterised by airway luminal Th17 pathway activation

Chen, Alice C.-H., Martin, Megan L., Lourie, Rohan, Rogers, Geraint B., Burr, Lucy D., Hasnain, Sumaira Z., Bowler, Simon D., McGuckin, Michael A. and Serisier, David J. (2015) Adult non-cystic fibrosis bronchiectasis is characterised by airway luminal Th17 pathway activation. PLoS ONE, 10 3: . doi:10.1371/journal.pone.0119325

Author Chen, Alice C.-H.
Martin, Megan L.
Lourie, Rohan
Rogers, Geraint B.
Burr, Lucy D.
Hasnain, Sumaira Z.
Bowler, Simon D.
McGuckin, Michael A.
Serisier, David J.
Title Adult non-cystic fibrosis bronchiectasis is characterised by airway luminal Th17 pathway activation
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2015-03-30
Year available 2015
Sub-type Article (original research)
DOI 10.1371/journal.pone.0119325
Open Access Status DOI
Volume 10
Issue 3
Total pages 13
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Collection year 2016
Language eng
Formatted abstract
Non-cystic fibrosis (CF) bronchiectasis is characterised by chronic airway infection and neutrophilic inflammation, which we hypothesised would be associated with Th17 pathway activation.

Th17 pathway cytokines were quantified in bronchoalveolar lavage fluid (BALF), and gene expression of IL-17A, IL-1β, IL-8 and IL-23 determined from endobronchial biopsies (EBx) in 41 stable bronchiectasis subjects and 20 healthy controls. Relationships between IL-17A levels and infection status, important clinical measures and subsequent Pseudomonas aeruginosa infection were determined.

BALF levels of all Th17 cytokines (median (IQR) pg/mL) were significantly higher in bronchiectasis than control subjects, including IL-17A (1.73 (1.19, 3.23) vs. 0.27 (0.24, 0.35), 95% CI 1.05 to 2.21, p<0.0001) and IL-23 (9.48 (4.79, 15.75) vs. 0.70 (0.43, 1.79), 95% CI 4.68 to 11.21, p<0.0001). However, BALF IL-17A levels were not associated with clinical measures or airway microbiology, nor predictive of subsequent P. aeruginosa infection. Furthermore, gene expression of IL-17A in bronchiectasis EBx did not differ from control. In contrast, gene expression (relative to medians of controls) in bronchiectasis EBx was significantly higher than control for IL1β (4.12 (1.24, 8.05) vs 1 (0.13, 2.95), 95% CI 0.05 to 4.07, p = 0.04) and IL-8 (3.75 (1.64, 11.27) vs 1 (0.54, 3.89), 95% CI 0.32 to 4.87, p = 0.02) and BALF IL-8 and IL-1α levels showed significant relationships with clinical measures and airway microbiology. P. aeruginosa infection was associated with increased levels of IL-8 while Haemophilus influenzae was associated with increased IL-1α.

Conclusions and Clinical Relevance
Established adult non-CF bronchiectasis is characterised by luminal Th17 pathway activation, however this pathway may be relatively less important than activation of non-antigen-specific innate neutrophilic immunity.
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Institutional Status UQ

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