Fluoromica nanoparticle cytotoxicity in macrophages decreases with size and extent of uptake

Tee, Nicolin, Zhu, Yingdong, Mortimer, Gysell M., Martin, Darren J. and Minchin, Rodney F. (2015) Fluoromica nanoparticle cytotoxicity in macrophages decreases with size and extent of uptake. International Journal of Nanomedicine, 10 2363-2375. doi:10.2147/IJN.S80655


Author Tee, Nicolin
Zhu, Yingdong
Mortimer, Gysell M.
Martin, Darren J.
Minchin, Rodney F.
Title Fluoromica nanoparticle cytotoxicity in macrophages decreases with size and extent of uptake
Journal name International Journal of Nanomedicine   Check publisher's open access policy
ISSN 1176-9114
1178-2013
Publication date 2015-01
Year available 2015
Sub-type Article (original research)
DOI 10.2147/IJN.S80655
Open Access Status DOI
Volume 10
Start page 2363
End page 2375
Total pages 13
Place of publication Macclesfield, United Kingdom
Publisher Dove Medical Press
Collection year 2016
Language eng
Formatted abstract
Polyurethanes are widely used in biomedical devices such as heart valves, pacemaker leads, catheters, vascular devices, and surgical dressings because of their excellent mechanical properties and good biocompatibility. Layered silicate nanoparticles can significantly increase tensile strength and breaking strain of polyurethanes potentially increasing the life span of biomedical devices that suffer from wear in vivo. However, very little is known about how these nanoparticles interact with proteins and cells and how they might exert unwanted effects. A series of fluoromica nanoparticles ranging in platelet size from 90 to over 600 nm in diameter were generated from the same base material ME100 by high energy milling and differential centrifugation. The cytotoxicity of the resulting particles was dependent on platelet size but in a manner that is opposite to many other types of nanomaterials. For the fluoromicas, the smaller the platelet size, the less toxicity was observed. The small fluoromica nanoparticles (<200 nm) were internalized by macrophages via scavenger receptors, which was dependent on the protein corona formed in serum. This internalization was associated with apoptosis in RAW cells but not in dTHP-1 cells. The larger particles were not internalized efficiently but mostly decorated the surface of the cells, causing membrane disruption, even in the presence of 80% serum. This work suggests the smaller fluoromica platelets may be safer for use in humans but their propensity to recognize macrophage scavenger receptors also suggests that they will target the reticulo-endoplasmic system in vivo.
Keyword Layered silicates
Accumulation
Phagocytosis
High energy milling
Silica Induced Apoptosis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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