Urokinase and urokinase receptor expression in somatic cell hybrids

Mazzieri, R, Hoyerhansen, G, Ronne, E, Lober, D, Vagnarelli, P, Raimondi, E, Decarli, L, Dano, K and Mignatti, P (1994) Urokinase and urokinase receptor expression in somatic cell hybrids. Fibrinolysis and Proteolysis, 8 6: 344-352. doi:10.1016/0268-9499(94)90002-7

Author Mazzieri, R
Hoyerhansen, G
Ronne, E
Lober, D
Vagnarelli, P
Raimondi, E
Decarli, L
Dano, K
Mignatti, P
Title Urokinase and urokinase receptor expression in somatic cell hybrids
Journal name Fibrinolysis and Proteolysis   Check publisher's open access policy
ISSN 0268-9499
Publication date 1994
Sub-type Article (original research)
DOI 10.1016/0268-9499(94)90002-7
Volume 8
Issue 6
Start page 344
End page 352
Total pages 9
Language eng
Subject 2720 Hematology
Abstract To study the mechanisms that control cell-bound urokinase (u-PA) activity we characterised the expression of u-PA and its cellular receptor (u-PAR) in somatic cell hybrids between mouse L fibroblasts that do not produce u-PA and human HT1080 fibrosarcoma cells that express high levels of u-PA and u-PAR. Although the hybrids possessed a number of copies of the human u-PA and u-PAR genes comparable to that of HT1080 cells, they had levels of human u-PA mRNA and u-PAR mRNA significantly lower than HT1080 cells. Accordingly, the levels of u-PA and u-PAR were lower in the hybrids than in HT1080 cells. However, the hybrid cells surprisingly had a 2- to 6-fold higher u-PA-binding capacity than HT1080 cells. Some hybrid cells expressed glycosilation variants of u-PAR. In addition, in the hybrids, that express very low levels of u-PA, u-PAR was predominantly in the ligand-binding, three-domain form, whereas HT1080 cells, that have a high u-PA level, possessed higher levels of the cleaved, two-domain form of u-PAR devoid of ligand-binding activity. This indicates that the u-PA-binding capacity is controlled not only by u-PAR expression but also depends on receptor affinity and cleavage by u-PA or plasmin.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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