A promoter from sugarcane bacilliform badnavirus drives transgene expression in banana and other monocot and dicot plants

Schenk, P. M., Sagi, L., Remans, T., Dietzgen, R. G., Bernard, M. J., Graham, M. W. and Manners, J. M. (1999) A promoter from sugarcane bacilliform badnavirus drives transgene expression in banana and other monocot and dicot plants. Plant Molecular Biology, 39 6: 1221-1230.


Author Schenk, P. M.
Sagi, L.
Remans, T.
Dietzgen, R. G.
Bernard, M. J.
Graham, M. W.
Manners, J. M.
Title A promoter from sugarcane bacilliform badnavirus drives transgene expression in banana and other monocot and dicot plants
Journal name Plant Molecular Biology   Check publisher's open access policy
ISSN 0167-4412
Publication date 1999-04
Sub-type Article (original research)
DOI 10.1023/A:1006125229477
Volume 39
Issue 6
Start page 1221
End page 1230
Total pages 10
Place of publication Dordrecht
Publisher Kluwer
Language eng
Abstract A 1369 bp DNA fragment (Sc) was isolated from a full-length clone of sugarcane bacilliform badnavirus (ScBV) and was shown to have promoter activity in transient expression assays using monocot (banana, maize, millet and sorghum) and dicot plant species (tobacco, sunflower, canola and Nicotiana benthamiana). This promoter was also tested for stable expression in transgenic banana and tobacco plants. These experiments showed that this promoter could drive high-level expression of the beta-glucuronidase (GUS) reporter gene in most plant cells. The expression level was comparable to the maize ubiquitin promoter in standardised transient assays in maize. In transgenic banana plants the expression levels were variable for different transgenic lines but was generally comparable with the activities of both the maize ubiquitin promoter and the enhanced cauliflower mosaic virus (CaMV) 35S promoter. The Sc promoter appears to express in a near-constitutive manner in transgenic banana and tobacco plants. The promoter from sugarcane bacilliform virus represents a useful tool for the high-level expression of foreign genes in both monocot and dicot transgenic plants that could be used similarly to the CaMV 35S or maize polyubiquitin promoter.
Keyword Biochemistry & Molecular Biology
Plant Sciences
Constitutive Expression
Gfp
Gus
Musa
Scbv
Transformation
Mottle Virus Promoter
Gene-expression
Monocotyledonous Plants
Mosaic-virus
Rice
Dna
Transformation
Elements
Tobacco
Identification
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Mon, 13 Aug 2007, 11:06:44 EST