A robust tissue culture system for sorghum [Sorghum bicolor (L.) Moench]

Liu, Guoquan, Gilding, Edward K and Godwin, Ian D (2015) A robust tissue culture system for sorghum [Sorghum bicolor (L.) Moench]. South African Journal of Botany, 98 157-160. doi:10.1016/j.sajb.2015.03.179


Author Liu, Guoquan
Gilding, Edward K
Godwin, Ian D
Title A robust tissue culture system for sorghum [Sorghum bicolor (L.) Moench]
Journal name South African Journal of Botany   Check publisher's open access policy
ISSN 0254-6299
1727-9321
Publication date 2015-05
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.sajb.2015.03.179
Open Access Status
Volume 98
Start page 157
End page 160
Total pages 4
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2016
Language eng
Formatted abstract
Sorghum tissue culture has been challenged by three predominant obstacles for decades, namely toxic pigments (phenolics), low regeneration frequencies and short duration of callus regenerability. Here, we report a robust tissue culture system for sorghum, which has minimized these major impediments. To optimize media, different concentrations of various plant growth regulators, such as 2,4-dichlorophenoxyacetic acid (2,4-D), N6-benzyladenine (BA), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and α-naphthaleneacetic acid (NAA) were evaluated. Additional ingredients, including KH2PO4, CuSO4·5H2O, l-asparagine, l-proline and polyvinylpyrrolidone (PVP) were also assessed. Results showed that callus age had a conspicuous effect on its growth and regenerability, with callus weekly growth ratio and regenerability peaked at two weeks after induction. A callus induction rate up to 100% was achieved in inbred line Tx430, whereas regeneration rates up to 100% were obtained from SA281 and 91419R. This highly efficient system has been utilized for sorghum transformation for several years and has been proven to be reliable and reproducible.
Keyword Plant growth regulator
Immature embryo
Copper sulfate
Callus age
Callus regenerability
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2016 Collection
Institute for Molecular Bioscience - Publications
 
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