Impact of brief and sequential exposure to nystatin on the germ tube formation and cell surface hydrophobicity of oral Candida albicans isolates from human immunodeficiency virus-infected patients

Ellepola, Arjuna N. B. and Samaranayake, Lakshman P. (2014) Impact of brief and sequential exposure to nystatin on the germ tube formation and cell surface hydrophobicity of oral Candida albicans isolates from human immunodeficiency virus-infected patients. Medical Principles and Practice, 23 4: 307-312. doi:10.1159/000362369


Author Ellepola, Arjuna N. B.
Samaranayake, Lakshman P.
Title Impact of brief and sequential exposure to nystatin on the germ tube formation and cell surface hydrophobicity of oral Candida albicans isolates from human immunodeficiency virus-infected patients
Formatted title
Impact of brief and sequential exposure to nystatin on the germ tube formation and cell surface hydrophobicity of oral Candida albicans isolates from human immunodeficiency virus-infected patients
Journal name Medical Principles and Practice   Check publisher's open access policy
ISSN 1423-0151
1011-7571
Publication date 2014-07
Year available 2014
Sub-type Article (original research)
DOI 10.1159/000362369
Open Access Status DOI
Volume 23
Issue 4
Start page 307
End page 312
Total pages 6
Place of publication Basel, Switzerland
Publisher S. Karger AG
Collection year 2015
Language eng
Formatted abstract
Objective: To evaluate the impact of brief and sequential exposure to nystatin on the germ tube formation and cell surface hydrophobicity of oral isolates of Candida albicans obtained from patients infected with human immunodeficiency virus (HIV). Materials and Methods: After determining the minimum inhibitory concentration of nystatin, 10 oral isolates of C. albicans from 10 different HIV-infected patients were briefly (1 h) and sequentially (10 days) exposed to subtherapeutic concentrations of nystatin. Following a subsequent drug removal, the germ tube formation and cell surface hydrophobicity of these isolates were determined via a germ tube induction assay and an aqueous hydrocarbon assay, respectively. The data obtained from these assays for the control (unexposed to nystatin) and nystatin-exposed isolates were analyzed using Student's t tests. Results: The mean percentage reduction in the germ tube formation and cell surface hydrophobicity of the nystatin-exposed isolates compared to the controls was 30.12 ± 1.99 (p < 0.001) and 29.65 ± 2.33 (p < 0.001), respectively. Conclusion: These data elucidate the possible pharmacodynamic mechanisms by which nystatin might operate in vivo in the modulation of candidal virulence.
Keyword Candida albicans
Cell surface hydrophobicity
Germ tubes
Nystatin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Dentistry Publications
 
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Created: Tue, 31 Mar 2015, 12:16:23 EST by Margot Dallinger on behalf of School of Dentistry