Preventing root caries development under oral biofilm challenge in an artificial mouth

Mei, May-Lei, Chu, Chun-Hung, Lo, Edward-Chin-Man and Samaranayake, Lakshman-Perera (2013) Preventing root caries development under oral biofilm challenge in an artificial mouth. Medicina Oral, Patologia Oral y Cirugia Bucal, 18 4: E557-E563. doi:10.4317/medoral.18768

Author Mei, May-Lei
Chu, Chun-Hung
Lo, Edward-Chin-Man
Samaranayake, Lakshman-Perera
Title Preventing root caries development under oral biofilm challenge in an artificial mouth
Journal name Medicina Oral, Patologia Oral y Cirugia Bucal   Check publisher's open access policy
ISSN 1698-4447
Publication date 2013-07-01
Year available 2013
Sub-type Article (original research)
DOI 10.4317/medoral.18768
Open Access Status
Volume 18
Issue 4
Start page E557
End page E563
Total pages 7
Place of publication Valencia, Spain
Publisher Medicina Oral Sl
Collection year 2014
Language eng
Subject 3500 Dentistry
2733 Otorhinolaryngology
2746 Surgery
Abstract Objectives: To study the preventive effects of chlorhexidine against root caries under oral biofilm in an artificial mouth. Study Design: Sixteen human tooth-root disks were inoculated with a salivary sample that was produced by mixing the unstimulated saliva of three adults who had no untreated caries. The disks were incubated in an artificial mouth fed with a 5% sucrose solution three times daily for one week. Eight disks received a twice daily rinse of 0.12% chlorhexidine (test group). The other eight disks were rinsed in distilled water (control). The biofilm was then studied with three techniques: colony forming unit (CFU) counting, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The changes in the chemical structure of the root surface were studied using Fourier transform infra-Red spectroscopy. Type-I collagen and proteoglycans on the root surface were quantified using immunocytochemical staining. Results: The log CFU for the test and control groups were 4.21 and 8.27, respectively (p<0.001). The CFU count of Streptococci and Lactobacilli were negligible. Both the SEM and the CLSM showed suppressed bacteria growth in the test group. The log [amide-I: HPO4 2-] of the test and control groups were 1.11 and 1.93, respectively (p=0.02). The mean counts of sound type-I collagen in the test and control groups were 16.8/μn2 and 13.0/μn2, respectively (p<0.001), whereas the mean counts of intact proteoglycans were 5.6/μm2 and 3.5/μm2, respectively (P<0.001). Conclusions: Chlorhexidine suppressed the growth of selected cariogenic bacteria in oral biofilm on the root surface and thus protected tooth-root from cariogenic challenge.
Keyword Artificial mouth
Caries risk
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
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Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
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Created: Tue, 31 Mar 2015, 12:00:57 EST by Margot Dallinger on behalf of School of Dentistry