Antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries

Mei, May Lei, Li, Quan-li, Chu, Chun-Hung, Lo, Edward Chin-Man and Samaranayake, Lakshman Perera (2013) Antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries. Annals of Clinical Microbiology and Antimicrobials, 12 4: . doi:10.1186/1476-0711-12-4

Author Mei, May Lei
Li, Quan-li
Chu, Chun-Hung
Lo, Edward Chin-Man
Samaranayake, Lakshman Perera
Title Antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries
Journal name Annals of Clinical Microbiology and Antimicrobials   Check publisher's open access policy
ISSN 1476-0711
Publication date 2013-02-26
Year available 2013
Sub-type Article (original research)
DOI 10.1186/1476-0711-12-4
Open Access Status DOI
Volume 12
Issue 4
Total pages 7
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2014
Language eng
Abstract Backgrounds: Silver diamine fluoride (SDF) has clinical success in arresting dentin caries, this study aimed to investigate its mechanism of action. Methods: Using a computer-controlled artificial mouth, we studied the effect of 38% SDF on cariogenic biofilms and dentin carious lesions. We used five common cariogenic bacteria (Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, Lactobacillus rhamnosus and Actinomyces naeslundii) to form a cariogenic biofilm that generated carious lesions with a depth of approximately 70 um on human dentin blocks. We applied 38% SDF to the lesions in the test group and water to those in the control group. The blocks were incubated in the artificial mouth for 21 days before evaluation. Microbial kinetics, architecture, viability and distribution were evaluated every 7 days using colony forming unit (CFU), scanning electron microscopy and confocal laser scanning microscopy. The physical properties of the carious lesions were evaluated with microhardness testing, energy dispersive spectroscopy (EDS) and Fourier transform infra-red spectroscopy (FTIR). Results: The CFU results revealed fewer colony forming units in the test group compared with the control group (p < 0.01). Scanning electron microscopy and confocal microscopy showed less bacterial growth in the test group, and confluent cariogenic biofilm in the control group (p < 0.01). The microhardness and weight percentages of calcium and phosphorus in the test group from the outermost 50mum were higher than in the control group (p < 0.05). EDS showed that calcium and phosphous were higher in outer 50 mum in test groups than in the control FTIR revealed less exposed collagen I in the test lesions compared with the control group (p < 0.01). Conclusions: 38% SDF inhibits multi-species cariogenic biofilm formation on dentin carious lesions and reduces the demineralization process.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
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Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 31 Mar 2015, 11:44:02 EST by Margot Dallinger on behalf of School of Dentistry