Exopolysaccharide matrix of developed Candida albicans biofilms after exposure to antifungal agents

Da Silva, Wander Jose, Goncalves, Letícia Machado, Seneviratne, Jayampath, Parahitiyawa, Nipuna, Samaranayake, Lakshman Perera and Del Bel Cury, Altair Antoninha (2012) Exopolysaccharide matrix of developed Candida albicans biofilms after exposure to antifungal agents. Brazilian Dental Journal, 23 6: 716-722. doi:10.1590/S0103-64402012000600016

Author Da Silva, Wander Jose
Goncalves, Letícia Machado
Seneviratne, Jayampath
Parahitiyawa, Nipuna
Samaranayake, Lakshman Perera
Del Bel Cury, Altair Antoninha
Title Exopolysaccharide matrix of developed Candida albicans biofilms after exposure to antifungal agents
Journal name Brazilian Dental Journal   Check publisher's open access policy
ISSN 0103-6440
Publication date 2012
Year available 2012
Sub-type Article (original research)
DOI 10.1590/S0103-64402012000600016
Open Access Status DOI
Volume 23
Issue 6
Start page 716
End page 722
Total pages 7
Place of publication Riberao Preto, Brazil
Publisher Fundacao Odontologica de Ribeirao Preto
Language eng
Formatted abstract
This study aimed to evaluate the effects of fluconazole or nystatin exposure on developed Candida albicans biofilms regarding their exopolysaccharide matrix. The minimal inhibitory concentration (MIC) against fluconazole or nystatin was determined for C. albicans reference strain (ATCC 90028). Poly(methlymethacrylate) resin (PMMA) specimens were fabricated according to the manufacturer's instructions and had their surface roughness measured. Biofilms were developed on specimens surfaces for 48 h and after that were exposed during 24 h to fluconazole or nystatin prepared in a medium at MIC, 10 x MIC or 100 x MIC. Metabolic activity was evaluated using an XTT assay. Production of soluble and insoluble exopolysaccharide and intracellular polysaccharides was evaluated by the phenol-sulfuric method. Confocal laser scanning microscope was used to evaluate biofilm architecture and percentage of dead/live cells. Data were analyzed statistically by ANOVA and Tukey's test at 5% significance level. The presence of fluconazole or nystatin at concentrations higher than MIC results in a great reduction of metabolic activity (p<0.001). At MIC or 10 x MIC, fluconazole showed high amounts of intracellular polysaccharides (p<0.05), but did not affect the exopolysaccharide matrix (p>0.05). The exposure to nystatin also did not alter the exopolysaccharide matrix at all the tested concentrations (p>0.05). Biofilm architecture was not affected by either of the antifungal agents (p>0.05). Nystatin promoted higher proportion of dead cells (p<0.05). It may be concluded that fluconazole and nystatin above the MIC concentration reduced the metabolic activity of C. albicans biofilms; however, they were not able to alter the exopolysaccharide matrix and biofilm architecture.
Keyword Antifungal agents
Candida albicans
Denture bases
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
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Created: Tue, 31 Mar 2015, 11:42:18 EST by Margot Dallinger on behalf of School of Dentistry