Human serum potentiates the expression of genes associated with antifungal drug resistance in C. albicans biofilms on central venous catheters

Samaranayake, L. P., Anil, S., Hashem, M., Vellappally, S. and Cheung, B. P. K. (2015) Human serum potentiates the expression of genes associated with antifungal drug resistance in C. albicans biofilms on central venous catheters. Mycopathologia, 179 3-4: 195-204. doi:10.1007/s11046-014-9848-z


Author Samaranayake, L. P.
Anil, S.
Hashem, M.
Vellappally, S.
Cheung, B. P. K.
Title Human serum potentiates the expression of genes associated with antifungal drug resistance in C. albicans biofilms on central venous catheters
Formatted title
Human serum potentiates the expression of genes associated with antifungal drug resistance in C. albicans biofilms on central venous catheters
Journal name Mycopathologia   Check publisher's open access policy
ISSN 0301-486X
1573-0832
Publication date 2015-04-01
Year available 2014
Sub-type Article (original research)
DOI 10.1007/s11046-014-9848-z
Volume 179
Issue 3-4
Start page 195
End page 204
Total pages 10
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Collection year 2015
Language eng
Formatted abstract
Candida albicans is a major agent of fungaemias and frequently causes systemic disease through seeded, blood stream dissemination. These infections, particularly common in hospitalized patients with central venous catheters (CVCs), appear to persevere due to biofilm reservoirs of the yeast that tend to develop on the device. Although it is known that candidal biofilms are intrinsically resistant to antifungals compared with their planktonic counterparts, there is a paucity of data on the expression of antifungal drug resistance genes (DRGs) in candidal biofilms in CVC reservoirs. Furthermore, notwithstanding the fact that CVCs are constantly bathed in human serum, there are no studies on the effect of the latter on the DRG expression in candidal biofilms. Hence, we developed in vitro biofilms of three different C. albicans strains on silicone CVC discs immersed in human serum and evaluated the temporal expression of nine antifungal DRGs. In an attempt to evaluate the effect of hyphal elements on DRG expression, we incorporated a hyphal mutant (HM) and its wild-type (WT) counterpart, as well as a fresh clinical isolate in the studies. Human serum significantly up-regulated DRG transcripts in Candida biofilms on CVCs, at different stages of biofilm growth, while the WT strain over-expressed more DRGs than the HM strain. Here, we report, for the first time, that both human serum and the hyphal elements of the yeast have a profound modulatory effect on DRG expression in C. albicans biofilms on CVCs.
Keyword Serum
Drug resistance genes
C. albicans biofilms
Central venous catheters
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 17 Dec 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
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