The impact of acute lung injury, ECMO and transfusion on oxidative stress and plasma selenium levels in an ovine model

McDonald, Charles I, Fung, Yoke Lin, Shekar, Kiran, Diab, Sara D, Dunster, Kimble R, Passmore, Margaret R, Foley, Samuel R, Simonova, Gabriela, Platts, David and Fraser, John F (2015) The impact of acute lung injury, ECMO and transfusion on oxidative stress and plasma selenium levels in an ovine model. Journal of Trace Elements in Medicine and Biology, 30 4-10. doi:10.1016/j.jtemb.2015.01.004

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Author McDonald, Charles I
Fung, Yoke Lin
Shekar, Kiran
Diab, Sara D
Dunster, Kimble R
Passmore, Margaret R
Foley, Samuel R
Simonova, Gabriela
Platts, David
Fraser, John F
Title The impact of acute lung injury, ECMO and transfusion on oxidative stress and plasma selenium levels in an ovine model
Journal name Journal of Trace Elements in Medicine and Biology   Check publisher's open access policy
ISSN 1878-3252
0946-672X
Publication date 2015-04
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.jtemb.2015.01.004
Open Access Status File (Author Post-print)
Volume 30
Start page 4
End page 10
Total pages 7
Place of publication Muenchen, Germany
Publisher Elsevier GmbH - Urban und Fischer
Collection year 2016
Language eng
Formatted abstract
The purpose of this study was to determine the effects of smoke induced acute lung injury (S-ALI), extracorporeal membrane oxygenation (ECMO) and transfusion on oxidative stress and plasma selenium levels. Forty ewes were divided into (i) healthy control (n = 4), (ii) S-ALI control (n = 7), (iii) ECMO control (n = 7), (iv) S-ALI + ECMO (n = 8) and (v) S-ALI + ECMO + packed red blood cell (PRBC) transfusion (n = 14). Plasma thiobarbituric acid reactive substances (TBARS), selenium and glutathione peroxidase (GPx) activity were analysed at baseline, after smoke injury (or sham) and 0.25, 1, 2, 6, 7, 12 and 24 h after initiation of ECMO. Peak TBARS levels were similar across all groups. Plasma selenium decreased by 54% in S-ALI sheep (1.36 ± 0.20 to 0.63 ± 0.27 μmol/L, p < 0.0001), and 72% in sheep with S-ALI + ECMO at 24 h (1.36 ± 0.20 to 0.38 ± 0.19, p < 0.0001). PRBC transfusion had no effect on TBARS, selenium levels or glutathione peroxidase activity in plasma. While ECMO independently increased TBARS in healthy sheep to levels which were similar to the S-ALI control, the addition of ECMO after S-ALI caused a negligible increase in TBARS. This suggests that the initial lung injury was the predominant feature in the TBARS response. In contrast, the addition of ECMO in S-ALI sheep exacerbated reductions in plasma selenium beyond that of S-ALI or ECMO alone. Clinical studies are needed to confirm the extent and duration of selenium loss associated with ECMO.
Keyword Extracorporeal membrane oxygenation
Acute lung injury
Selenium
Oxidative stress
Antioxidants
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Medicine Publications
 
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