Microdialysis measurements of lamellar perfusion and energy metabolism during the development of laminitis in the oligofructose model

Medina-Torres, C. E., Underwood, C., Pollitt, C. C., Castro-Olivera, E. M., Hodson, M. P., Richardson, D. W. and Van Eps, A. W. (2015) Microdialysis measurements of lamellar perfusion and energy metabolism during the development of laminitis in the oligofructose model. Equine Veterinary Journal, 48 2: 246-252. doi:10.1111/evj.12417


Author Medina-Torres, C. E.
Underwood, C.
Pollitt, C. C.
Castro-Olivera, E. M.
Hodson, M. P.
Richardson, D. W.
Van Eps, A. W.
Title Microdialysis measurements of lamellar perfusion and energy metabolism during the development of laminitis in the oligofructose model
Journal name Equine Veterinary Journal   Check publisher's open access policy
ISSN 2042-3306
0425-1644
Publication date 2015
Year available 2015
Sub-type Article (original research)
DOI 10.1111/evj.12417
Open Access Status Not Open Access
Volume 48
Issue 2
Start page 246
End page 252
Total pages 7
Place of publication Hoboken NJ, United States
Publisher John Wiley & Sons
Collection year 2016
Language eng
Formatted abstract
Reasons for performing study
Failure of lamellar energy metabolism, with or without ischaemia, may be important in the pathophysiology of sepsis-associated laminitis.

Objectives
To examine lamellar perfusion and energy balance during laminitis development in the oligofructose model using tissue microdialysis.

Study design
In vivo experiment.

Methods
Six Standardbred horses underwent laminitis induction using the oligofructose model (OFT group) and 6 horses were untreated controls (CON group). Microdialysis probes were placed in the lamellar tissue of one forelimb (all horses) as well as the skin dermis of the tail in OFT horses. Dialysate and plasma samples were collected every 2 h for 24 h and concentrations of energy metabolites (glucose, lactate, pyruvate) and standard indices of energy metabolism (lactate to glucose ratio [L:G] and lactate to pyruvate ratio [L:P]) determined. Microdialysis urea clearance was used to estimate changes in tissue perfusion. Data were analysed nonparametrically.

Results
Median glucose concentration decreased to <30% of baseline by 8 h in OFT lamellar (P = <0.01) and skin (P<0.01) dialysate. Lactate increased mildly in skin dialysate (P = 0.04) and plasma (P = 0.05) but not lamellar dialysate in OFT horses. Median pyruvate concentration decreased to <50% of baseline in OFT lamellar dialysate (P = 0.03). A >5-fold increase in median L:G compared with baseline occurred in OFT lamellar and skin dialysate (P<0.03). From a baseline of <20, median L:P increased to a peak of 80 in OFT skin and 38.7 in OFT lamellar dialysates (P<0.02); however, OFT lamellar dialysate L:P was not significantly different from CON. Urea concentration decreased significantly in OFT lamellar dialysate (increased urea clearance) but not in OFT skin or CON lamellar dialysate.

Conclusions
Increased lamellar perfusion occurred during the development of sepsis-associated laminitis in the oligofructose model. Glucose concentrations in the lamellar interstitium decreased, suggesting increased glucose consumption but there was no definitive evidence of lamellar energy failure.
Keyword Horse
Bioenergetics
Microdialysis
Metabolite
Oligofructose
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Wed, 08 Jul 2015, 15:03:18 EST