Activity-driven relaxation of the cortical actomyosin II network synchronizes Munc18-1-dependent neurosecretory vesicle docking

Papadopulos, Andreas, Gomez, Guillermo A., Martin, Sally, Jackson, Jade, Gormal, Rachel S., Keating, Damien J., Yap, Alpha S. and Meunier, Frederic A. (2015) Activity-driven relaxation of the cortical actomyosin II network synchronizes Munc18-1-dependent neurosecretory vesicle docking. Nature Communications, 6 6297: 1-11. doi:10.1038/ncomms7297

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Author Papadopulos, Andreas
Gomez, Guillermo A.
Martin, Sally
Jackson, Jade
Gormal, Rachel S.
Keating, Damien J.
Yap, Alpha S.
Meunier, Frederic A.
Title Activity-driven relaxation of the cortical actomyosin II network synchronizes Munc18-1-dependent neurosecretory vesicle docking
Journal name Nature Communications   Check publisher's open access policy
ISSN 2041-1723
Publication date 2015-02-25
Year available 2015
Sub-type Article (original research)
DOI 10.1038/ncomms7297
Open Access Status File (Publisher version)
Volume 6
Issue 6297
Start page 1
End page 11
Total pages 11
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2016
Language eng
Formatted abstract
In neurosecretory cells, secretory vesicles (SVs) undergo Ca2+-dependent fusion with the plasma membrane to release neurotransmitters. How SVs cross the dense mesh of the cortical actin network to reach the plasma membrane remains unclear. Here we reveal that, in bovine chromaffin cells, SVs embedded in the cortical actin network undergo a highly synchronized transition towards the plasma membrane and ​Munc18-1-dependent docking in response to secretagogues. This movement coincides with a translocation of the cortical actin network in the same direction. Both effects are abolished by the knockdown or the pharmacological inhibition of myosin II, suggesting changes in actomyosin-generated forces across the cell cortex. Indeed, we report a reduction in cortical actin network tension elicited on secretagogue stimulation that is sensitive to myosin II inhibition. We reveal that the cortical actin network acts as a ‘casting net’ that undergoes activity-dependent relaxation, thereby driving tethered SVs towards the plasma membrane where they undergo ​Munc18-1-dependent docking.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2016 Collection
Institute for Molecular Bioscience - Publications
 
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