Reconstruction of Cyclooxygenase Evolution in Animals Suggests Variable, Lineage-Specific Duplications, and Homologs with Low Sequence Identity

Havird, Justin C, Kocot, Kevin M, Brannock, Pamela M, Cannon, Johanna T, Waits, Damien S, Weese, David A, Santos, Scott R and Halanych, Kenneth M (2015) Reconstruction of Cyclooxygenase Evolution in Animals Suggests Variable, Lineage-Specific Duplications, and Homologs with Low Sequence Identity. Journal of Molecular Evolution, 80 3-4: 193-208. doi:10.1007/s00239-015-9670-3


Author Havird, Justin C
Kocot, Kevin M
Brannock, Pamela M
Cannon, Johanna T
Waits, Damien S
Weese, David A
Santos, Scott R
Halanych, Kenneth M
Title Reconstruction of Cyclooxygenase Evolution in Animals Suggests Variable, Lineage-Specific Duplications, and Homologs with Low Sequence Identity
Journal name Journal of Molecular Evolution   Check publisher's open access policy
ISSN 0022-2844
1432-1432
Publication date 2015-04
Year available 2015
Sub-type Article (original research)
DOI 10.1007/s00239-015-9670-3
Open Access Status
Volume 80
Issue 3-4
Start page 193
End page 208
Total pages 16
Place of publication New York, United States
Publisher Springer New York LLC
Abstract Cyclooxygenase (COX) enzymatically converts arachidonic acid into prostaglandin G/H in animals and has importance during pregnancy, digestion, and other physiological functions in mammals. COX genes have mainly been described from vertebrates, where gene duplications are common, but few studies have examined COX in invertebrates. Given the increasing ease in generating genomic data, as well as recent, although incomplete descriptions of potential COX sequences in Mollusca, Crustacea, and Insecta, assessing COX evolution across Metazoa is now possible. Here, we recover 40 putative COX orthologs by searching publicly available genomic resources as well as ~250 novel invertebrate transcriptomic datasets. Results suggest the common ancestor of Cnidaria and Bilateria possessed a COX homolog similar to those of vertebrates, although such homologs were not found in poriferan and ctenophore genomes. COX was found in most crustaceans and the majority of molluscs examined, but only specific taxa/lineages within Cnidaria and Annelida. For example, all octocorallians appear to have COX, while no COX homologs were found in hexacorallian datasets. Most species examined had a single homolog, although species-specific COX duplications were found in members of Annelida, Mollusca, and Cnidaria. Additionally, COX genes were not found in Hemichordata, Echinodermata, or Platyhelminthes, and the few previously described COX genes in Insecta lacked appreciable sequence homology (although structural analyses suggest these may still be functional COX enzymes). This analysis provides a benchmark for identifying COX homologs in future genomic and transcriptomic datasets, and identifies lineages for future studies of COX.
Keyword Prostaglandin synthesis
Transcriptomics
Phylogenetics
Metazoa
Gene duplication and loss
Annelida
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Biological Sciences Publications
 
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