Rumen function in vivo and in vitro in sheep fed Leucaena leucocephala

Barros-Rodriguez, Marcos Antonio, Solorio-Sanchez, Francisco Javier, Sandoval-Castro, Carlos Alfredo, Klieve, Athol, Rojas-Herrera, Rafael Antonio, Briceno-Poot, Eduardo G. and Ku-Vera, Juan Carlos (2015) Rumen function in vivo and in vitro in sheep fed Leucaena leucocephala. Tropical Animal Health and Production, 47 4: 757-764. doi:10.1007/s11250-015-0790-y

Author Barros-Rodriguez, Marcos Antonio
Solorio-Sanchez, Francisco Javier
Sandoval-Castro, Carlos Alfredo
Klieve, Athol
Rojas-Herrera, Rafael Antonio
Briceno-Poot, Eduardo G.
Ku-Vera, Juan Carlos
Title Rumen function in vivo and in vitro in sheep fed Leucaena leucocephala
Formatted title
Rumen function in vivo and in vitro in sheep fed Leucaena leucocephala
Journal name Tropical Animal Health and Production   Check publisher's open access policy
ISSN 1573-7438
Publication date 2015-04
Year available 2015
Sub-type Article (original research)
DOI 10.1007/s11250-015-0790-y
Open Access Status
Volume 47
Issue 4
Start page 757
End page 764
Total pages 8
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Collection year 2016
Language eng
Formatted abstract
The effect of Leucaena leucocephala inclusion in sheep diets upon rumen function was evaluated. Nine Pelibuey sheep, 32.6 ± 5.33 kg live weight (LW), fitted with rumen cannula were used. A complete randomized block design was employed. Two experimental periods of 60 days each, with 60-day intervals between them, were used. Experimental treatments were as follows (n = 6): T1 (control), 100 % Pennisetum purpureum grass; T2, 20 % L. leucocephala + 80 % P. purpureum; T3, 40 % L. leucocephala + 60 % P. purpureum. In situ rumen neutral detergent fiber (aNDF) and crude protein (CP) degradation, dry matter intake (DMI), volatile fatty acids (VFA) production, estimated methane (CH4) yield, rumen pH, ammonia nitrogen (N-NH3), and protozoa counts were measured. The aNDF in situ rumen degradation of P. purpureum and leucaena was higher (P < 0.05) in T2 and T3. Leucaena CP degradation was higher in T2 and T3 but for P. purpureum it was only significantly higher in T3. Leucaena aNDF and CP degradation rate (c) was 50 % higher (P < 0.05) in T2 and T3, but only higher in T3 for P. purpureum. Voluntary intake and rumen (N-NH3) was higher in T2 and T3 (P = 0.0001, P = 0.005, respectively). Molar VFA proportions were similar for all treatments (P > 0.05). Protozoa counts and in vitro gas production (48 h) were lower in T2 and T3 (P < 0.05, P < 0.0001). Estimated methane yield (mol CH4/day) was higher in sheep fed leucaena (P < 0.0001). However, CH4 yield relative to animal performance (mol CH4/g LW gain) was lower in T2 and T3 (P < 0.0001). In summary, these results indicate that including L. leucocephala in sheep diets did not modify rumen fermentation pattern (same VFA ratios) nor reduce the amount of CH4 per unit of DMI (mol CH4/g DMI). However, leucaena inclusion does increase rumen N-NH3, aNDF and CP digestibility, and voluntary intake.
Keyword Tropical fodder
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2016 Collection
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