QCM-based rapid analysis of DNA

Dultsev, F. N., Kolosovsky, E. A., Cooper, M. A., Lomzov, A. A. and Pyshnyi, D. V. (2015) QCM-based rapid analysis of DNA. Sensing and Bio-Sensing Research, 4 11-15. doi:10.1016/j.sbsr.2014.10.004


Author Dultsev, F. N.
Kolosovsky, E. A.
Cooper, M. A.
Lomzov, A. A.
Pyshnyi, D. V.
Title QCM-based rapid analysis of DNA
Journal name Sensing and Bio-Sensing Research   Check publisher's open access policy
ISSN 2214-1804
Publication date 2015-06-01
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.sbsr.2014.10.004
Open Access Status DOI
Volume 4
Start page 11
End page 15
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2016
Language eng
Abstract We demonstrated that it is possible to break apart double-stranded DNA using a thickness shear mode resonator. This procedure measures the force of bond rupture. The measured rupture forces were in the range of 10–40 pN. The procedure was tested experimentally with model complementary and non-complementary oligonucleotides. In the case of the DNA mismatch we observed a signal at a driving voltage of 0.8 V (corresponding to a shear force of 11 pN), while in the case of complementarity the signal at the voltage of 3 V (36 pN) appeared. This procedure may be the ground for the development of new types of biosensors measuring the force of affine interactions (probe/biomolecular target) on the surface of the sensor element.
Keyword Thickness shear mode resonator
DNA
Bond rupture
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Institute for Molecular Bioscience - Publications
 
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