Herpes simplex virus 1 targets the murine olfactory neuroepithelium for host entry

Shivkumar, Maitreyi, Milho, Ricardo, May, Janet S., Nicoll, Michael P., Efstathiou, Stacey and Stevenson, Philip G. (2013) Herpes simplex virus 1 targets the murine olfactory neuroepithelium for host entry. Journal of Virology, 87 19: 10477-10488. doi:10.1128/JVI.01748-13

Author Shivkumar, Maitreyi
Milho, Ricardo
May, Janet S.
Nicoll, Michael P.
Efstathiou, Stacey
Stevenson, Philip G.
Title Herpes simplex virus 1 targets the murine olfactory neuroepithelium for host entry
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
Publication date 2013-10
Year available 2013
Sub-type Article (original research)
DOI 10.1128/JVI.01748-13
Open Access Status DOI
Volume 87
Issue 19
Start page 10477
End page 10488
Total pages 12
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2013
Language eng
Formatted abstract
Herpes simplex virus 1 (HSV-1) is a ubiquitous and important human pathogen. It is known to persist in trigeminal ganglia (TG), but how it reaches this site has been difficult to determine, as viral transmission is sporadic, pathogenesis is complicated, and early infection is largely asymptomatic. We used mice to compare the most likely natural HSV-1 host entry routes: oral and nasal. Intranasal infection was 100-fold more efficient than oral and targeted predominantly the olfactory neuroepithelium. Live imaging of HSV-1-expressed luciferase showed infection progressing from the nose to the TG and then reemerging in the facial skin. The brain remained largely luciferase negative throughout. Infected cell tagging by viral Cre recombinase expression in floxed reporter gene mice showed nasal virus routinely reaching the TG and only rarely reaching the olfactory bulbs. Thus, HSV-1 spread from the olfactory neuroepithelium to the TG and reemerged peripherally without causing significant neurological disease. This recapitulation of typical clinical infection suggests that HSV-1 might sometimes also enter humans via the respiratory tract.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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Created: Mon, 23 Mar 2015, 11:48:22 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences