In-vitro evaluation of ion-exchange microspheres for the sustained release of liposomal-adenoviral conjugates

Steel, JC, Cavanagh, HMA, Burton, MA, Dingwall, D and Kalle, WHJ (2004) In-vitro evaluation of ion-exchange microspheres for the sustained release of liposomal-adenoviral conjugates. Journal of Controlled Release, 95 3: 601-611. doi:10.1016/j.jconrel.2004.01.001


Author Steel, JC
Cavanagh, HMA
Burton, MA
Dingwall, D
Kalle, WHJ
Title In-vitro evaluation of ion-exchange microspheres for the sustained release of liposomal-adenoviral conjugates
Journal name Journal of Controlled Release   Check publisher's open access policy
ISSN 0168-3659
Publication date 2004-03-24
Sub-type Article (original research)
DOI 10.1016/j.jconrel.2004.01.001
Volume 95
Issue 3
Start page 601
End page 611
Total pages 11
Language eng
Subject 3003 Pharmaceutical Science
Abstract This study looks at the development of a novel combination vector consisting of adenovirus conjugated to liposomes (AL complexes) bound to cation-exchanging microspheres (MAL complexes). With adenovirus having a net negative charge and the liposomes a net positive charge it was possible to modify the net charge of the AL complexes by varying the concentrations of adenovirus to liposomes. The modification of the net charge resulted in altered binding and release characteristics. Of the complexes tested, the 5:1 and 2:1 ratio AL complexes were able to be efficiently bound by the microspheres and exhibited sustained release over 24 h. The 1:1 and 1:2 AL complexes, however, bound poorly to the microspheres and were rapidly released. In addition the MAL complexes also were able to reduce the toxicity of the AL complexes, which was seen with the 10:1 ratio. The AL complexes showed considerably more toxicity alone than in combination with microspheres, highlighting a potential benefit of this vector.
Keyword AL and MAL complexes
Ion-exchange microspheres
Liposomal-adenoviral conjugates
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 10 times in Scopus Article | Citations
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Created: Tue, 17 Mar 2015, 16:09:26 EST by Jason Steel