Quantitative PCR measurements of Escherichia coli including shiga toxin-producing E. coli (STEC) in animal feces and environmental waters

Ahmed W., Gyawali, Pradip and Toze, Simon (2015) Quantitative PCR measurements of Escherichia coli including shiga toxin-producing E. coli (STEC) in animal feces and environmental waters. Environmental Science and Technology, 49 5: 3084-3090. doi:10.1021/es505477n


Author Ahmed W.
Gyawali, Pradip
Toze, Simon
Title Quantitative PCR measurements of Escherichia coli including shiga toxin-producing E. coli (STEC) in animal feces and environmental waters
Journal name Environmental Science and Technology   Check publisher's open access policy
ISSN 1520-5851
0013-936X
Publication date 2015-02-03
Year available 2015
Sub-type Article (original research)
DOI 10.1021/es505477n
Open Access Status
Volume 49
Issue 5
Start page 3084
End page 3090
Total pages 7
Place of publication Washington, United States
Publisher American Chemical Society
Collection year 2016
Language eng
Formatted abstract
Quantitative PCR (qPCR) assays were used to determine the concentrations of E. coli including shiga toxin-producing E. coli (STEC) associated virulence genes (eaeA, stx1, stx2, and hlyA) in ten animal species (fecal sources) and environmental water samples in Southeast Queensland, Australia. The mean Log10 concentrations and standard deviations of E. coli 23S rRNA across fecal sources ranged from 1.3 ± 0.1 (horse) to 6.3 ± 0.4 (cattle wastewater) gene copies at a test concentration of 10 ng of DNA. The differences in mean concentrations of E. coli 23S rRNA gene copies among fecal source samples were significantly different from each other (P < 0.0001). Among the virulence genes, stx2 (25%, 95% CI, 17–33%) was most prevalent among fecal sources, followed by eaeA (19%, 95% CI, 12–27%), stx1 (11%, 95% CI, 5%–17%) and hlyA (8%, 95% CI, 3–13%). The Log10 concentrations of STEC virulence genes in cattle wastewater samples ranged from 3.8 to 5.0 gene copies at a test concentration of 10 ng of DNA. Of the 18 environmental water samples tested, three (17%) were positive for eaeA and two (11%) samples were also positive for the stx2 virulence genes. The data presented in this study will aid in the estimation of quantitative microbial risk assessment (QMRA) from fecal pollution of domestic and wild animals in drinking/recreational water catchments.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Public Health Publications
 
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