Actinobacillus pleuropneumoniae: an update from Down Under

Turni, Conny, Singh, Reema, Omaleki, Lida and Blackall, Pat (2014). Actinobacillus pleuropneumoniae: an update from Down Under. In: Pasteurella 2014: International Pasteurellaceae Conference 2014, Prato, Italy, (). 13-16 May, 2014.

Author Turni, Conny
Singh, Reema
Omaleki, Lida
Blackall, Pat
Title of paper Actinobacillus pleuropneumoniae: an update from Down Under
Formatted title
Actinobacillus pleuropneumoniae – an update from Down Under
Conference name Pasteurella 2014: International Pasteurellaceae Conference 2014
Conference location Prato, Italy
Conference dates 13-16 May, 2014
Publication Year 2014
Sub-type Published abstract
Open Access Status
Language eng
Formatted Abstract/Summary
Actinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia and contributes to substantial economic losses in the swine industry worldwide. There are currently a total of 15 serovars recognised. In recent years we have observed submissions from farms previously free of pleuropneumonia. We have also had an increase in submissions of Actinobacillus-like isolates. In this study the prevalence of A. pleuropneumoniae serovars over 11 years and the occurrence of related species were investigated. The study also examined the hypothesis that serovar 5 isolates had limited genetic diversity. The 336 isolates of App sumitted from February 2002 until April 2013 were serotyping via gel diffusion, indirect haemagglutination and multiplex PCR. Genotyping of the serovar 5 isolates was performed with the enterobacterial repetitive intergenic consensus (ERIC) PCR. Partial sequencing of the 16S rDNA gene and blast analysis was done to determine the species of the Actinobacillus-like isolates, with the gene being first amplified with primers 27F, 519R, 530F and 1525R. There appears to have been a shift in serovars in 2012 with serovar 12 submissions from outbreaks being more prominent than in previous years. When determining the genotype of serovar 5 isolates in the period from 2002 to 2013, all 27 isolates, originating from 11 farms from three Australian States, displayed the same genetic fingerprint. This suggests that the isolates are a single clone and of one origin. Twenty-eight Actinobacillus-like isolates were submitted in the period of October 2011 until November 2012. Thirteen of these isolates had the same ERIC profile, yet four of them were identified (minimum of 96% identity) as A. indolicus, six as A. porcitonsillarum and three as A. porcitonsillarum/minor complex. Further investigations into the Actinobacillus-like isolates that have emerged in the last year are necessary to define the species of these isolates.
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Other title: "Actinobacillus pleuropneumoniae: a diagnostic update".

Document type: Conference Paper
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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Created: Mon, 16 Mar 2015, 22:30:54 EST by Dr Patrick Blackall on behalf of Centre for Animal Science