Crystallization of importin alpha, the nuclear-import receptor

Teh, T, Tiganis, T and Kobe, B (1999) Crystallization of importin alpha, the nuclear-import receptor. Acta Crystallographica Section D: Biological Crystallography, 55 2: 561-563. doi:10.1107/S0907444998012943

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Author Teh, T
Tiganis, T
Kobe, B
Title Crystallization of importin alpha, the nuclear-import receptor
Journal name Acta Crystallographica Section D: Biological Crystallography   Check publisher's open access policy
ISSN 0907-4449
Publication date 1999
Sub-type Article (original research)
DOI 10.1107/S0907444998012943
Open Access Status File (Publisher version)
Volume 55
Issue 2
Start page 561
End page 563
Total pages 3
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell
Language eng
Abstract Crystals of recombinant importin alpha, the nuclear-import receptor, have been obtained at two different pH conditions by vapour diffusion using sodium citrate as precipitant and dithiothreitol as an additive. At pH 4-5, the crystals have the symmetry of the trigonal space group P3(1)21 or P3(2)21 (a = b = 78.0, c = 255.8 Angstrom, gamma = 120 degrees); at pH 6-7, the crystals have the symmetry of the orthorhombic space group P2(1)2(1)2(1) (a = 78.5, b = 89.7, c = 100.5 Angstrom). In both cases, there is probably one molecule of importin ct in the asymmetric unit. At least one of the crystal forms diffracts to a resolution higher than 3 Angstrom using the laboratory X-ray source; the crystals are suitable for crystal structure determination.
Keyword Biochemical Research Methods
Biochemistry & Molecular Biology
Sv40 T-antigen
Protein Import
Localization Sequence
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
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Created: Mon, 13 Aug 2007, 10:53:35 EST