Impact of brief and sequential exposure to nystatin, amphotericin B, ketoconazole, and fluconazole in modulating adhesion traits of oral Candida dubliniensis isolates

Ellepola, Arjuna N. B., Joseph, Bobby K., Samaranayake, Lakshman P., Bandara, H. M. H. N. and Khan, Zia U. (2014) Impact of brief and sequential exposure to nystatin, amphotericin B, ketoconazole, and fluconazole in modulating adhesion traits of oral Candida dubliniensis isolates. Journal of Investigative and Clinical Dentistry, . doi:10.1111/jicd.12132


Author Ellepola, Arjuna N. B.
Joseph, Bobby K.
Samaranayake, Lakshman P.
Bandara, H. M. H. N.
Khan, Zia U.
Title Impact of brief and sequential exposure to nystatin, amphotericin B, ketoconazole, and fluconazole in modulating adhesion traits of oral Candida dubliniensis isolates
Formatted title
Impact of brief and sequential exposure to nystatin, amphotericin B, ketoconazole, and fluconazole in modulating adhesion traits of oral Candida dubliniensis isolates
Journal name Journal of Investigative and Clinical Dentistry   Check publisher's open access policy
ISSN 2041-1626
2041-1618
Publication date 2014-11-11
Year available 2014
Sub-type Article (original research)
DOI 10.1111/jicd.12132
Open Access Status
Total pages 9
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell Publishing
Collection year 2015
Language eng
Formatted abstract
Aim
Candida adherence is implicated in the pathogenesis of oral candidosis. Adhesion to buccal epithelial cells (BEC), germ tube (GT) formation, and relative cell surface hydrophobicity (CSH) are colonization attributes of candidal pathogenicity. Candida dubliniensis (C. dubliniensis) is allied with recurrent oral candidosis, which can be treated with nystatin, amphotericin B, ketoconazole, and fluconazole. Due to the diluent effect of saliva and the cleansing effect of the oral musculature in the oral cavity C. dubliniensis isolates undergo brief and sequential exposure to antifungal agents during therapy. Thus, in the present study, we evaluated the adhesion to BEC, GT formation, and the CSH of oral isolates of C. dubliniensis following brief and sequential exposure to nystatin, amphotericin B, ketoconazole, and fluconazole.

Methods
After determining the minimum inhibitory concentration (MIC) of the aforementioned drugs, 20 oral isolates of C. dubliniensis were briefly (1 h), and sequentially (10 days) exposed to subcidal concentrations of these drugs. Following drug removal, adhesion to BEC, GT formation, and CSH of these isolates were determined.

Results
The percentage reduction of adhesion to BEC, GT formation, and CSH of the isolates following exposure to antifungal agents were as follows: nystatin: 53.55%, 33.98%, and 29.83% (P < 0.001); amphotericin B: 53.84%, 36.23%, and 28.97% (P < 0.001); ketoconazole: 37.43%, 20.51%, and 16.49% (P < 0.001); and fluconazole: 8.93% (P < 0.001), 1.6%, and 0.63% (P > 0.05).

Conclusions
Brief and sequential exposure of C. dubliniensis to antifungal agents would continue to wield an antifungal effect by altering its adhesion attributes, and elucidate possible pharmacodynamics by which antifungal agents might operate in modulating candidal adherence.
Keyword Adhesion
Antifungal agent
Candida dubliniensis
Oral candidosis
Polyene
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print, 11 November 2014.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Dentistry Publications
 
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Created: Wed, 11 Mar 2015, 13:04:50 EST by Margot Dallinger on behalf of School of Dentistry