High-resolution DNA melt-curve analysis for cost-effective mass screening of pairwise species interactions

McCarthy, James K., Didham, Raphael K., Brockerhoff, Eckehard G., van Bysterveldt, Katherine A. and Varsani, Arvind (2013) High-resolution DNA melt-curve analysis for cost-effective mass screening of pairwise species interactions. Molecular Ecology Resources, 13 5: 908-917. doi:10.1111/1755-0998.12143

Author McCarthy, James K.
Didham, Raphael K.
Brockerhoff, Eckehard G.
van Bysterveldt, Katherine A.
Varsani, Arvind
Title High-resolution DNA melt-curve analysis for cost-effective mass screening of pairwise species interactions
Journal name Molecular Ecology Resources   Check publisher's open access policy
ISSN 1755-098X
Publication date 2013
Year available 2013
Sub-type Article (original research)
DOI 10.1111/1755-0998.12143
Open Access Status
Volume 13
Issue 5
Start page 908
End page 917
Total pages 10
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Abstract Ecological studies of pairwise interactions are constrained by the methods available for rapid species identification of the interacting organisms. The resolution of data required to characterize species interaction networks at multiple spatio-temporal scales can be intensive, and therefore laborious and costly to collect. We explore the utility of high-resolution DNA melt-curve analysis (HRM) as a rapid species identification method. An approach was developed to identify organisms at the pairwise interaction level, with particular application to cryptic species interactions that are traditionally difficult to study. Here, we selected a challenging application; to identify the presence/absence of pathogenic fungi (Sporothrix inflata, Ophiostoma nigrocarpum and Ophiostoma galeiforme) transported by bark beetle vectors (Hylastes ater and Hylurgus ligniperda). The technique was able to distinguish between different species of DNA within a single, pooled sample. In test applications, HRM was effective in the mass screening and identification of pathogenic fungal species carried by many individual bark beetle vectors (n = 455 beetles screened) across large geographic scales. For two of the fungal species, there was no difference in the frequency of association with either of their vectors, but for the third fungal species there was a shift in vector-pathogen associations across locations. This technique allows rapid, mass screening and characterization of species interactions at a fraction of the time and cost of traditional methods. It is anticipated that this method can be readily applied to explore other cryptic species interactions, or other studies requiring rapid generation of large data sets and/or high-throughput efficiency.
Keyword Bark beetle
Insect vectoring
Interaction network
Rapid species identification
Sapstain fungi
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 09 Mar 2015, 08:39:40 EST by James McCarthy on behalf of School of Biological Sciences