How deeply does your mutant sleep? Probing arousal to better understand sleep defects in Drosophila

Faville, R., Kottler, B., Goodhill, G. J., Shaw, P. J. and van Swinderen, B. (2015) How deeply does your mutant sleep? Probing arousal to better understand sleep defects in Drosophila. Scientific Reports, 5 Art No.: 8454: . doi:10.1038/srep08454


Author Faville, R.
Kottler, B.
Goodhill, G. J.
Shaw, P. J.
van Swinderen, B.
Title How deeply does your mutant sleep? Probing arousal to better understand sleep defects in Drosophila
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2015-02
Year available 2015
Sub-type Article (original research)
DOI 10.1038/srep08454
Open Access Status DOI
Volume 5
Issue Art No.: 8454
Total pages 13
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2016
Language eng
Formatted abstract
The fruitfly, Drosophila melanogaster, has become a critical model system for investigating sleep functions. Most studies use duration of inactivity to measure sleep. However, a defining criterion for sleep is decreased behavioral responsiveness to stimuli. Here we introduce the Drosophila ARousal Tracking system (DART), an integrated platform for efficiently tracking and probing arousal levels in animals. This video-based platform delivers positional and locomotion data, behavioral responsiveness to stimuli, sleep intensity measures, and homeostatic regulation effects – all in one combined system. We show how insight into dynamically changing arousal thresholds is crucial for any sleep study in flies. We first find that arousal probing uncovers different sleep intensity profiles among related genetic background strains previously assumed to have equivalent sleep patterns. We then show how sleep duration and sleep intensity can be uncoupled, with distinct manipulations of dopamine function producing opposite effects on sleep duration but similar sleep intensity defects. We conclude by providing a multi-dimensional assessment of combined arousal and locomotion metrics in the mutant and background strains. Our approach opens the door for deeper insights into mechanisms of sleep regulation and provides a new method for investigating the role of different genetic manipulations in controlling sleep and arousal.
Keyword Synaptic Homeostasis
Dopaminergic Neurons
Regulates Sleep
Melanogaster
Wakefulness
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Mathematics and Physics
Queensland Brain Institute Publications
Official 2016 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
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