Evidence of multiple transcription initiation and termination sites within the rDNA intergenic spacer and rRNA readthrough transcription in the urochordate Herdmania curvata

Degnan, Bernard M., Yan, Jun and Lavin, Martin F. (1998) Evidence of multiple transcription initiation and termination sites within the rDNA intergenic spacer and rRNA readthrough transcription in the urochordate Herdmania curvata. Molecular Marine Biology And Biotechnology, 7 4: 294-302.


Author Degnan, Bernard M.
Yan, Jun
Lavin, Martin F.
Title Evidence of multiple transcription initiation and termination sites within the rDNA intergenic spacer and rRNA readthrough transcription in the urochordate Herdmania curvata
Journal name Molecular Marine Biology And Biotechnology   Check publisher's open access policy
ISSN 1053-6426
Publication date 1998-12-01
Sub-type Article (original research)
Volume 7
Issue 4
Start page 294
End page 302
Total pages 9
Place of publication New York
Publisher Springer
Language eng
Subject 11 Medical and Health Sciences
Abstract Analysis of the structure of the urochordate Herdmania curvata ribosomal DNA intergenic spacer (IGS) and its role in transcription initiation and termination suggests that rRNA gene regulation in this chordate differs from that in vertebrates. A cloned H, curvata IGS is 1881 bp and composed predominantly of two classes of similar repeat sequences that largely alternate in a tandem array. Southern blot hybridization demonstrates that the IGS length variation within an individual and population is largely the result of changes in internal repeat number. Nuclease S1 mapping and primer extension analyses suggest that there are two transcription initiation sites at the 3' end of the most 3' repetitive element; these sites are 6 nucleotides apart. Unlike mouse, Xenopus, and Drosophila, there is no evidence of transcription starting elsewhere in the IGS. Most sequence differences between the promoter repeat and the other internal repeats are in the vicinity of the putative initiation sites. As in Drosophila, nuclease S1 mapping of transcription termination sites suggest that there is not a definitive stop site and a majority of the pre-rRNAs read through a substantial portion of the IGS. Some transcription appears to proceed completely through the promoter repeat into the adjacent rDNA unit. Analysis of oocyte RNA by reverse transcription-polymerase chain reaction (RT-PCR) confirms that readthrough transcription into the adjacent rDNA unit is occurring in some small IGS length variants; there is no evidence of complete readthrough of IGSs larger than 1.0 kb.
Keyword Biotechnology & Applied Microbiology
Marine & Freshwater Biology
Ribosomal-rna Genes
Xenopus-laevis
Polymerase-i
Dna
Momus
Mechanisms
Eukaryotes
Sequence
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
School of Biological Sciences Publications
 
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Created: Mon, 13 Aug 2007, 20:50:34 EST