Interfacial biomimetic synthesis of silica nanocapsules using a recombinant catalytic modular protein

Wibowo, David, Zhao, Chun-Xia and Middelberg, Anton P. J. (2015) Interfacial biomimetic synthesis of silica nanocapsules using a recombinant catalytic modular protein. Langmuir, 31 6: 1999-2007. doi:10.1021/la504684g


Author Wibowo, David
Zhao, Chun-Xia
Middelberg, Anton P. J.
Title Interfacial biomimetic synthesis of silica nanocapsules using a recombinant catalytic modular protein
Journal name Langmuir   Check publisher's open access policy
ISSN 1520-5827
0743-7463
Publication date 2015-02-17
Year available 2015
Sub-type Article (original research)
DOI 10.1021/la504684g
Open Access Status
Volume 31
Issue 6
Start page 1999
End page 2007
Total pages 9
Place of publication Washington, DC United States
Publisher American Chemical Society
Collection year 2016
Language eng
Formatted abstract
This paper reports interfacially driven synthesis of oil-core silica-shell nanocapsules using a rationally designed recombinant catalytic modular protein (ReCaMoP), in lieu of a conventional chemical surfactant. A 116-residue protein, D4S2, was designed by modularizing a surface-active protein module having four-helix bundle structure in bulk and a biosilicification-active peptide module rich in cationic residues. This modular combination design allowed the protein to be produced via the industrially relevant cell factory Escherichia coli with simplified purification conferred by thermostability engineered in design. Dynamic interfacial tension and thin film pressure balance were used to gain an overview of the protein behavior at macroscopic interfaces. Functionalities of D4S2 to make silica nanocapsules were demonstrated by facilitating formation and stabilization of pharmaceutically grade oil droplets through its surface-active module and then by directing nucleation and growth of a silica shell at the oil–water interface through its biosilicification-active module. Through these synergistic activities in D4S2, silica nanocapsules could be formed at near-neutral pH and ambient temperature without using any organic solvents that might have negative environmental and sustainability impacts. This work introduces parallelization of biomolecular, scale-up and interfacial catalytic design strategies for the ultimate development of sustainable and scalable production of a recombinant modular protein that is able to catalyze synthesis of oil-filled silica nanocapsules under environmentally friendly conditions, suitable for use as controlled-release nanocarriers of various actives in biomedical and agricultural applications.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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