Glucose conjugation of anti-HIV-1 oligonucleotides containing unmethylated CpG motifs reduces their immunostimulatory activity

Reyes-Darias, Jose A., Sanchez-Luque, Francisco J., Morales, Juan Carlos, Perez-Rentero, Sonia, Eritja, Ramon and Berzal-Herranz, Alfredo (2015) Glucose conjugation of anti-HIV-1 oligonucleotides containing unmethylated CpG motifs reduces their immunostimulatory activity. ChemBioChem, 16 4: 584-591. doi:10.1002/cbic.201402574


Author Reyes-Darias, Jose A.
Sanchez-Luque, Francisco J.
Morales, Juan Carlos
Perez-Rentero, Sonia
Eritja, Ramon
Berzal-Herranz, Alfredo
Title Glucose conjugation of anti-HIV-1 oligonucleotides containing unmethylated CpG motifs reduces their immunostimulatory activity
Journal name ChemBioChem   Check publisher's open access policy
ISSN 1439-7633
1439-4227
Publication date 2015-03-02
Year available 2015
Sub-type Article (original research)
DOI 10.1002/cbic.201402574
Open Access Status
Volume 16
Issue 4
Start page 584
End page 591
Total pages 8
Place of publication Weinheim, Germany
Publisher Wiley
Collection year 2016
Language eng
Abstract Antisense oligodeoxynucleotides (ODNs) are short synthetic DNA polymers complementary to a target RNA sequence. They are commonly designed to halt a biological event, such as translation or splicing. ODNs are potentially useful therapeutic agents for the treatment of different human diseases. Carbohydrate–ODN conjugates have been reported to improve the cell-specific delivery of ODNs through receptor mediated endocytosis. We tested the anti-HIV activity and biochemical properties of the 5′-end glucose-conjugated GEM 91 ODN targeting the initiation codon of the gag gene of HIV-1 RNA in cell-based assays. The conjugation of a glucose residue significantly reduces the immunostimulatory effect without diminishing its potent anti-HIV-1 activity. No significant effects were observed in either ODN stability in serum, in vitro degradation of antisense DNA–RNA hybrids by RNase H, cell toxicity, cellular uptake and ability to interfere with genomic HIV-1 dimerisation.
Keyword Antiviral agents
Glycoconjugates
HIV-1 inhibition
Immune response reduction
Oligonucleotides
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Health Services Publications
Non HERDC
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 2 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 03 Mar 2015, 00:19:41 EST by System User on behalf of Mater Research Institute-UQ