Resistance of Black-lip learl oyster, Pinctada margaritifera, to infection by Ostreid herpes virus 1(mu)var under experimental challenge may be mediated by humoral antiviral activity

Tan, Terence L. S., Paul-Pont, Ika, Evans, Olivia M., Watterson, Daniel, Young, Paul, Whittington, Richard, Fougerouse, Angelique, Bichet, Hervé, Barnes, Andrew C. and Dang, Cécile (2015) Resistance of Black-lip learl oyster, Pinctada margaritifera, to infection by Ostreid herpes virus 1(mu)var under experimental challenge may be mediated by humoral antiviral activity. Fish and Shellfish Immunology, 44 1: 232-240. doi:10.1016/j.fsi.2015.02.026

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads

Author Tan, Terence L. S.
Paul-Pont, Ika
Evans, Olivia M.
Watterson, Daniel
Young, Paul
Whittington, Richard
Fougerouse, Angelique
Bichet, Hervé
Barnes, Andrew C.
Dang, Cécile
Title Resistance of Black-lip learl oyster, Pinctada margaritifera, to infection by Ostreid herpes virus 1(mu)var under experimental challenge may be mediated by humoral antiviral activity
Formatted title
Resistance of Black-lip learl oyster, Pinctada margaritifera, to infection by Ostreid herpes virus 1μvar under experimental challenge may be mediated by humoral antiviral activity
Journal name Fish and Shellfish Immunology   Check publisher's open access policy
ISSN 1050-4648
1095-9947
Publication date 2015-02-21
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.fsi.2015.02.026
Open Access Status
Volume 44
Issue 1
Start page 232
End page 240
Total pages 9
Place of publication Camden, London, United Kingdom
Publisher Academic Press
Collection year 2016
Language eng
Formatted abstract
Highlights
• Black lipped pearl oyster P.margaritifera are not susceptible to highly virulent OSHV1-μvar.
• Herpesvirus-inhibiting activity was demonstrated in the acellular fraction of the hemolymph from P. margaritifera.
P. margaritifera does not appear ot be an effective host/carrier for Ostreid herpesvirus 1 – μvar.

Ostreid herpesvirus 1 (OsHV-1) has induced mass mortalities of the larvae and spat of Pacific oysters, Crassostrea gigas, in Europe and, more recently, in Oceania. The production of pearls from the Black-lip pearl oyster, Pinctada margaritifera, represents the second largest source of income to the economies of French Polynesia and many Pacific Island nations that could be severely compromised in the event of a disease outbreak. Coincidentally with the occurrence of OsHV-1 in the southern hemisphere, C. gigas imported from New Zealand and France into French Polynesia tested positive for OsHV-1. Although interspecies viral transmission has been demonstrated, the transmissibility of OsHV-1 to P. margaritifera is unknown. We investigated the susceptibility of juvenile P. margaritifera to OsHV-1 μvar that were injected with tissue homogenates sourced from either naturally infected or healthy C. gigas. The infection challenge lasted 14 days post-injection (dpi) with sampling at 0, 1, 2, 3, 5, 7 and 14 days. Mortality rate, viral prevalence, and cellular immune responses in experimental animals were determined. Tissues were screened by light microscopy and TEM. Pacific oysters were also challenged and used as a positive control to validate the efficiency of OsHV-1 μvar infection. Viral particles and features such as marginated chromatin and highly electron dense nuclei were observed in C. gigas but not in P. margaritifera. Mortality rates and hemocyte immune parameters, including phagocytosis and respiratory burst, were similar between challenged and control P. margaritifera. Herpesvirus-inhibiting activity was demonstrated in the acellular fraction of the hemolymph from P. margaritifera, suggesting that the humoral immunity is critical in the defence against herpesvirus in pearl oysters. Overall, these results suggest that under the conditions of the experimental challenge, P. margaritifera was not sensitive to OsHV-1 μvar and was not an effective host/carrier. The nature and spectrum of activity of the humoral antiviral activity is worthy of further investigation.
Keyword Ostreid herpesvirus
Humoral immunity
Hemocytes
Pinctada
Herpes
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 21 February 2015

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 3 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 27 Feb 2015, 09:25:50 EST by Mrs Louise Nimwegen on behalf of School of Biological Sciences