Pseudomonas aeruginosa genotypes acquired by children with cystic fibrosis by age 5-years

Kidd, Timothy J., Ramsay, Kay A., Vidmar, Suzanna, Carlin, John B., Bell, Scott C., Wainwright, Claire E. and Grimwood, Keith (2015) Pseudomonas aeruginosa genotypes acquired by children with cystic fibrosis by age 5-years. Journal of Cystic Fibrosis, 14 3: 361-369. doi:10.1016/j.jcf.2014.12.007


Author Kidd, Timothy J.
Ramsay, Kay A.
Vidmar, Suzanna
Carlin, John B.
Bell, Scott C.
Wainwright, Claire E.
Grimwood, Keith
Title Pseudomonas aeruginosa genotypes acquired by children with cystic fibrosis by age 5-years
Journal name Journal of Cystic Fibrosis   Check publisher's open access policy
ISSN 1569-1993
1873-5010
Publication date 2015-01-03
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.jcf.2014.12.007
Volume 14
Issue 3
Start page 361
End page 369
Total pages 9
Place of publication Amsterdam, The Netherlands
Publisher Elsevier
Collection year 2016
Language eng
Formatted abstract
Background
We describe Pseudomonas aeruginosa acquisitions in children with cystic fibrosis (CF) aged ≤ 5-years, eradication treatment efficacy, and genotypic relationships between upper and lower airway isolates and strains from non-CF sources.

Methods
Of 168 CF children aged ≤ 5-years in a bronchoalveolar lavage (BAL)-directed therapy trial, 155 had detailed microbiological results. Overall, 201/271 (74%) P. aeruginosa isolates from BAL and oropharyngeal cultures were available for genotyping, including those collected before and after eradication therapy.

Results
Eighty-two (53%) subjects acquired P. aeruginosa, of which most were unique strains. Initial eradication success rate was 90%, but 36 (44%) reacquired P. aeruginosa, with genotypic substitutions more common in BAL (12/14) than oropharyngeal (3/11) cultures. Moreover, oropharyngeal cultures did not predict BAL genotypes reliably.

Conclusions
CF children acquire environmental P. aeruginosa strains frequently. However, discordance between BAL and oropharyngeal strains raises questions over upper airway reservoirs and how to best determine eradication in non-expectorating children.
Keyword Cystic fibrosis
Eradication
Genotyping
Infection
Pseudomonas aeruginosa
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Medicine Publications
Child Health Research Centre Publications
 
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