Robust methods to create ex vivo minimum deformation atlases for brain mapping

Janke, Andrew L. and Ullmann, Jeremy F. P. (2015) Robust methods to create ex vivo minimum deformation atlases for brain mapping. Methods, 73 18-26. doi:10.1016/j.ymeth.2015.01.005


Author Janke, Andrew L.
Ullmann, Jeremy F. P.
Title Robust methods to create ex vivo minimum deformation atlases for brain mapping
Formatted title
Robust methods to create ex vivo minimum deformation atlases for brain mapping
Journal name Methods   Check publisher's open access policy
ISSN 1095-9130
1046-2023
Publication date 2015-02-01
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.ymeth.2015.01.005
Volume 73
Start page 18
End page 26
Total pages 9
Place of publication Maryland Heights, MO United States
Publisher Academic Press
Collection year 2016
Language eng
Formatted abstract
Highly detailed ex vivo 3D atlases of average structure are of critical importance to neuroscience and its current push to understanding the global microstructure of the brain. Multiple single slice histology sections can no longer provide sufficient detail of inter-slice microstructure and lack out of plane resolution. Two ex vivo methods have emerged that can create such detailed models. High-field micro MRI with the addition of contrast media has allowed intact whole brain microstructure imaging with an isotropic resolution of 15 μm in mouse.

Blockface imaging has similarly evolved to a point where it is now possible to image an entire brain in a rigorous fashion with an out of plane resolution of 10 μm. Despite the destruction of the tissue as part of this process it allows a reconstructed model that is free from cutting artifacts. Both of these methods have been utilised to create minimum deformation atlases that are representative of the respective populations. The MDA atlases allow us unprecedented insight into the commonality and differences in microstructure in cortical structures in specific taxa. In this paper we provide an overview of how to create such MDA models from ex vivo data.
Keyword Brain atlas
Minimum deformation atlas
Ex vivo imaging
Registration
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Centre for Advanced Imaging Publications
 
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