Molecular surveillance for carbapenemase genes in carbapenem-resistant Pseudomonas aeruginosa in Australian patients with cystic fibrosis

Tai, Anna S., Kidd, Timothy J., Whiley, David M., Ramsay, Kay A., Buckley, Cameron, Bell, Scott C., The ACPinCF Investigator Group and David, Michael (2015) Molecular surveillance for carbapenemase genes in carbapenem-resistant Pseudomonas aeruginosa in Australian patients with cystic fibrosis. Pathology, 47 2: 156-160. doi:10.1097/PAT.0000000000000216

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Author Tai, Anna S.
Kidd, Timothy J.
Whiley, David M.
Ramsay, Kay A.
Buckley, Cameron
Bell, Scott C.
The ACPinCF Investigator Group
David, Michael
Title Molecular surveillance for carbapenemase genes in carbapenem-resistant Pseudomonas aeruginosa in Australian patients with cystic fibrosis
Formatted title
Molecular surveillance for carbapenemase genes in carbapenem-resistant Pseudomonas aeruginosa in Australian patients with cystic fibrosis
Journal name Pathology   Check publisher's open access policy
ISSN 0031-3025
1465-3931
Publication date 2015-02-01
Sub-type Article (original research)
DOI 10.1097/PAT.0000000000000216
Volume 47
Issue 2
Start page 156
End page 160
Total pages 5
Place of publication London, United Kingdom
Publisher Lippincott Williams and Wilkins
Collection year 2016
Language eng
Formatted abstract
The aim of this study was to assess the prevalence of acquired carbapenemase genes amongst carbapenem non-susceptible Pseudomonas aeruginosa isolates in Australian patients with cystic fibrosis (CF). Cross-sectional molecular surveillance for acquired carbapenemase genes was performed on CF P. aeruginosa isolates from two isolate banks comprising: (i) 662 carbapenem resistant P. aerugi-nosa isolates from 227 patients attending 10 geographically diverse Australian CF centres (2007-2009), and (ii) 519 P. aeruginosa isolates from a cohort of 173 adult patients attending one Queensland CF clinic in 2011. All 1189 P. aeruginosa isolates were tested by polymerase chain reaction (PCR) protocols targeting ten common carbapene-mase genes, as well the Class 1 integron intI1 gene and the aadB aminoglycoside resistance gene. No carbapenemase genes were identified among all isolates tested. The intI1 and aadB genes were frequently detected and were significantly associated with the AUST-02 strain (OR 24.6, 95% CI 9.3-65.6; p<0.0001) predominantly from Queensland patients. Despite the high prevalence of carbapenem resistance in P. aeruginosa in Australian patients with CF, no acquired carbapenemase genes were detected in the study, suggesting chromosomal mutations remain the key resistance mechanism in CF isolates. Systematic surveillance for carbapenemase-producing P. aeruginosa in CF by molecular surveillance is ongoing.
Keyword Carbapenemase
Cystic fibrosis
Integron
Metallo-beta-lactamase (MBL)
Pseudomonas aeruginosa
Real-time PCR
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Children's Medical Research Institute Publications
Official 2016 Collection
School of Medicine Publications
 
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