The use of a conformational cathepsin D-derived epitope for vaccine development against Schistosoma mansoni

Ahmad Fuaad, Abdullah A. H., Roubille, Romain, Pearson, Mark S., Pickering, Darren A., Loukas, Alex C., Skwarczynski, Mariusz and Toth, Istvan (2015) The use of a conformational cathepsin D-derived epitope for vaccine development against Schistosoma mansoni. Bioorganic and Medicinal Chemistry, . doi:10.1016/j.bmc.2015.01.033

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads

Author Ahmad Fuaad, Abdullah A. H.
Roubille, Romain
Pearson, Mark S.
Pickering, Darren A.
Loukas, Alex C.
Skwarczynski, Mariusz
Toth, Istvan
Title The use of a conformational cathepsin D-derived epitope for vaccine development against Schistosoma mansoni
Formatted title
The use of a conformational cathepsin D-derived epitope for vaccine development against Schistosoma mansoni
Journal name Bioorganic and Medicinal Chemistry   Check publisher's open access policy
ISSN 0968-0896
1464-3391
Publication date 2015-01-30
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.bmc.2015.01.033
Total pages 6
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Collection year 2016
Language eng
Formatted abstract
Schistosomiasis is caused by the infection from Schistosoma species. Among these, Schistosoma mansoni is one of the major species that infects millions of people worldwide. The use of praziquantel is effective in clearing the infestation but treatment of a large and widespread population in endemic areas is unsustainable. Thus, synergistic approach of using drug and vaccination can serve as an alternative to the current treatment. In this study, we have developed vaccine candidates that composed of three components: a B-cell epitope derived from S. mansoni cathepsin D protein (Sm-CatD) flanked by GCN4 helix promoting peptide; a promiscuous T-helper epitope (P25); and a lipid core peptide system, in attempt to develop self-adjuvanting vaccine candidates against the schistosome. Physicochemical properties of the vaccine candidates were analysed and antibodies to each construct were raised in BALB/c mice. The vaccine candidates were able to self-assemble into particles that induced high titres of IgG without the use of additional adjuvant. The antibody levels were comparable to that induced by peptide formulated with strong but toxic Freund’s adjuvant. The integration of a GCN4 sequence induced the helical conformation of the epitope, while the addition of the T helper peptide was very effective in inducing consistent IgG-specific antibodies response amongst mice. These findings are particularly encouraging for the development of efficient and immunogenic vaccine against schistosomiasis.
Keyword Nanoparticles
Subunit peptide-based vaccine
Lipid core peptide
Epitope modification
Peptide conformation
Self-assembly
Schistosomiasis
Schistosoma mansoni
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 30 January 2015

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
School of Pharmacy Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Fri, 20 Feb 2015, 11:04:58 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences