T-DNA tagging and characterisation of a novel meristem-specific promoter from tobacco

Mudge, S. R. and Birch, R. G. (1998) T-DNA tagging and characterisation of a novel meristem-specific promoter from tobacco. Australian Journal of Plant Physiology, 25 6: 637-643.

Author Mudge, S. R.
Birch, R. G.
Title T-DNA tagging and characterisation of a novel meristem-specific promoter from tobacco
Journal name Australian Journal of Plant Physiology   Check publisher's open access policy
ISSN 0310-7841
Publication date 1998
Sub-type Article (original research)
Volume 25
Issue 6
Start page 637
End page 643
Total pages 7
Language eng
Abstract We have evaluated T-DNA mediated plant promoter tagging, with a left-border-linked promoterless firefly luciferase (luc) construct, as a strategy for the isolation of novel plant promoters. In a population of approximately 300 transformed tobacco plants, IO lines showed LUC activity, including novel tissue-specific and developmental patterns of expression. One line, showing LUC activity only in the shoot and root apical meristems, was further characterised. Inverse PCR was used to amplify a 1.5 kb fragment of plant DNA flanking the single-copy T-DNA insertion in this line. With the exception of a 249 bp highly repetitive element, this sequence is present as a single copy in the tobacco genome, and is not homologous to any previously characterised DNA sequences. Sequence analysis revealed the presence of several motifs that may be involved in transcriptional regulation. Transgenic tobacco plants transformed with a transcriptional fusion of this putative promoter sequence to the beta-glucuronidase (uidA) reporter gene, showed GUS activity confined to the shoot tip and mature pollen. This promoter may be useful to direct the expression of genes controlling the transition to flowering, or genes to reduce losses due to pests and stresses damaging plant apical meristems.
Keyword Plant Sciences
Promoter Tagging
Shoot Tip
Luc Gene
Inverse Pcr
Tissue-specific Expression
Gene Fusions
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
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Created: Mon, 13 Aug 2007, 10:44:52 EST