In vitro combinatorial anticancer effects of 5-fluorouracil and curcumin loaded N,O-carboxymethyl chitosan nanoparticles toward colon cancer and in vivo pharmacokinetic studies

Anitha, A., Sreeranganathan, Maya, Chennazhi, Krishna Prasad, Lakshmanan, Vinoth-Kumar and Jayakumar, R. (2014) In vitro combinatorial anticancer effects of 5-fluorouracil and curcumin loaded N,O-carboxymethyl chitosan nanoparticles toward colon cancer and in vivo pharmacokinetic studies. European Journal of Pharmaceutics and Biopharmaceutics, 88 1: 238-251. doi:10.1016/j.ejpb.2014.04.017


Author Anitha, A.
Sreeranganathan, Maya
Chennazhi, Krishna Prasad
Lakshmanan, Vinoth-Kumar
Jayakumar, R.
Title In vitro combinatorial anticancer effects of 5-fluorouracil and curcumin loaded N,O-carboxymethyl chitosan nanoparticles toward colon cancer and in vivo pharmacokinetic studies
Journal name European Journal of Pharmaceutics and Biopharmaceutics   Check publisher's open access policy
ISSN 0939-6411
1873-3441
Publication date 2014-09-01
Sub-type Article (original research)
DOI 10.1016/j.ejpb.2014.04.017
Open Access Status
Volume 88
Issue 1
Start page 238
End page 251
Total pages 14
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2015
Language eng
Abstract Colon cancer is the third most leading causes of death due to cancer worldwide and the chemo drug 5-fluorouracil's (5-FU) applicability is limited due to its non-specificity, low bioavailability and overdose. The efficacy of 5-FU in colon cancer chemo treatment could be improved by nanoencapsulation and combinatorial approach. In the present study curcumin (CUR), a known anticancer phytochemical, was used in combination with 5-FU and the work focuses on the development of a combinatorial nanomedicine based on 5-FU and CUR in N,O-carboxymethyl chitosan nanoparticles (N,O-CMC NPs). The developed 5-FU-N,O-CMC NPs and CUR-N,O-CMC NPs were found to be blood compatible. The in vitro drug release profile in pH 4.5 and 7.4 showed a sustained release profile over a period of 4 days. The combined exposure of the nanoformulations in colon cancer cells (HT 29) proved the enhanced anticancer effects. In addition, the in vivo pharmacokinetic data in mouse model revealed the improved plasma concentrations of 5-FU and CUR which prolonged up to 72 h unlike the bare drugs. In conclusion, the 5-FU and CUR released from the N,O-CMC NPs produced enhanced anticancer effects in vitro and improved plasma concentrations under in vivo conditions.
Keyword N,O-carboxymethyl chitosan nanoparticles
5-Fluorouracil
Curcumin
Drug delivery
Combinatorial Approach
Colon cancer
Pharmacokinetics
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 12 Feb 2015, 11:31:27 EST by Anitha Sudheesh Kumar on behalf of School of Chemistry & Molecular Biosciences