Curcumin-loaded N, O-carboxymethyl chitosan nanoparticles for cancer drug delivery

Anitha, A., Maya, S., Deepa, N., Chennazhi, K. P., Nair, S. V. and Jayakumar, R. (2012) Curcumin-loaded N, O-carboxymethyl chitosan nanoparticles for cancer drug delivery. Journal of Biomaterials Science, Polymer Edition, 23 11: 1381-1400. doi:10.1163/092050611X581534

Author Anitha, A.
Maya, S.
Deepa, N.
Chennazhi, K. P.
Nair, S. V.
Jayakumar, R.
Title Curcumin-loaded N, O-carboxymethyl chitosan nanoparticles for cancer drug delivery
Journal name Journal of Biomaterials Science, Polymer Edition   Check publisher's open access policy
ISSN 0920-5063
Publication date 2012-05-08
Sub-type Article (original research)
DOI 10.1163/092050611X581534
Open Access Status
Volume 23
Issue 11
Start page 1381
End page 1400
Total pages 20
Place of publication Abingdon, Oxfordshire, United Kingdom
Publisher Taylor & Francis
Language eng
Formatted abstract
Chitosan (CS) and its carboxymethyl derivatives are smart biopolymers that are non-toxic, biocompatible and biodegradable, and, hence, suitable for various biomedical applications, such as drug delivery, gene therapy and tissue engineering. Curcumin is a major chemotherapeutic agent with antioxidant, anti-inflammatory, anti-proliferative, anticancer and antimicrobial effects. However, the potential of curcumin as a chemotherapeutic agent is limited by its hydrophobicity and poor bioavailability. In this work, we developed a nanoformulation of curcumin in a carboxymethyl chitosan (CMC) derivative, N,O-carboxymethyl chitosan (N,O-CMC). The curcumin-loaded N,O-CMC (curcumin-N,O-CMC) nanoparticles were characterized using DLS, AFM, SEM, FT-IR and XRD. DLS studies revealed nanoparticles with a mean diameter of 150 ± 30 nm. AFM and SEM confirmed that the particles have a spherical morphology within the size range of 150 ± 30 nm. Curcumin was entrapped with in N,O-CMC nanopartcles with an efficiency of 80%. The in vitro drug-release profile was studied at different pH (7.4 and 4.5) at 37°C for different incubation periods with and without lysozyme. Cytotoxicity studies using MTT assay indicated that curcumin-N,O-CMC nanoparticles showed specific toxicity towards cancer cells and non-toxicity to normal cells. Cellular uptake of curcumin-N,O-CMC nanoparticles was analyzed by fluorescence microscopy and was reconfirmed by flow cytometry. Overall, these results indicate that like previously reported curcumin loaded O-CMC nanoparticles, N,O-CMC will also be an efficient nanocarrier for delivering curcumin to cancer cells.
Keyword N,O-Carboxymethyl chitosan
Cellular uptake
Cancer drug delivery
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 12 Feb 2015, 11:24:07 EST by Anitha Sudheesh Kumar on behalf of School of Chemistry & Molecular Biosciences