Acetylcholinesterase cDNA of the cattle tick, Boophilus microplus: characterisation and role in organophosphate resistance

Baxter, GD and Barker, SC (1998) Acetylcholinesterase cDNA of the cattle tick, Boophilus microplus: characterisation and role in organophosphate resistance. Insect Biochemistry And Molecular Biology, 28 8: 581-589.


Author Baxter, GD
Barker, SC
Title Acetylcholinesterase cDNA of the cattle tick, Boophilus microplus: characterisation and role in organophosphate resistance
Journal name Insect Biochemistry And Molecular Biology   Check publisher's open access policy
ISSN 0965-1748
Publication date 1998
Sub-type Article (original research)
DOI 10.1016/S0965-1748(98)00034-4
Volume 28
Issue 8
Start page 581
End page 589
Total pages 9
Language eng
Abstract Acetylcholinesterase is the target of organophosphate and carbamate pesticides. Organophosphate resistance is widespread in the cattle tick, Boophilus microplus, in Australia. We have isolated a cDNA of acetylcholinesterase from B. microplus and show that it would encode a protein 62 kDa in size. The predicted amino acid sequence contains all the residues characteristic of an acetylcholinesterase. Alternative splicing of the transcript was detected at both the 5' and 3' ends. Alternative splicing at the 5' end would result in two proteins differing by six amino acids. This is the first report of alternative splicing of the N-terminal coding region in a cholinesterase. No point mutations were detected in the acetylcholinesterase gene from organophosphate resistant strains of B. microplus. Alternative explanations for resistance to organophosphates in B. microplus are discussed. (C) 1998 Elsevier Science Ltd. All rights reserved.
Keyword Biochemistry & Molecular Biology
Entomology
Acetylcholinesterase
Cattle Tick
Organophosphate Resistance
Insecticide
Cdna Cloning
Alternative Splicing
Sodium-channel Gene
Nematode Caenorhabditis-elegans
Colorado Potato Beetle
Culex-pipiens Diptera
Insecticide Resistance
Insensitive Acetylcholinesterase
Gaba Receptor
Encoding Acetylcholinesterase
Azinphosmethyl Resistance
Pyrethroid Insecticides
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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