Metabolic flux analysis : methods and protocols

Metabolic flux analysis : methods and protocols. Edited by Jens O. Kromer, Lars Nielsen and Lars M. Blank New York, NY United States: Humana Press, 2014. doi:10.1007/978-1-4939-1170-7

Title Metabolic flux analysis : methods and protocols
Place of Publication New York, NY United States
Publisher Humana Press
Publication year 2014
Sub-type Reference work, encyclopaedia, manual or handbook
DOI 10.1007/978-1-4939-1170-7
Open Access Status
Volume 1191
Series Methods in Molecular Biology
ISBN 9781493911707
ISSN 1064-3745
Language eng
Editor Jens O. Kromer
Lars Nielsen
Lars M. Blank
Total number of pages 316
Collection year 2015
Year available 2014
Formatted Abstract/Summary
Metabolic Flux Analysis: Methods and Protocols opens up the field of metabolic flux analysis to those who want to start a new flux analysis project but are overwhelmed by the complexity of the approach. Metabolic flux analysis emerged from the current limitation for the prediction of metabolic fluxes from a measured inventory of the cell. Divided into convenient thematic parts, topics in this essential volume include the fundamental characteristics of the underlying networks, the application of quantitative metabolite data and thermodynamic principles to constrain the solution space for flux balance analysis (FBA), the experimental toolbox to conduct different types of flux analysis experiments, the processing of data from 13C experiments and three chapters that summarize some recent key findings. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Metabolic Flux Analysis: Methods and Protocols presents protocols that cover a range of relevant organisms currently used in the field, providing a solid basis to anybody interested in the field of metabolic flux analysis.
Q-Index Code AX
Q-Index Status Provisional Code
Institutional Status Non-UQ

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Created: Mon, 09 Feb 2015, 11:18:50 EST by Cathy Fouhy on behalf of Aust Institute for Bioengineering & Nanotechnology