Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing

Naveed, Muhammad, Mubeen, Samavia, Khan, SamiUllah, Ahmed, Iftikhar, Khalid, Nauman, Suleria, Hafiz Ansar Rasul, Bano, Asghari and Mumtaz, Abdul Samad (2014) Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing. Brazilian Journal of Microbiology, 45 3: 985-993.

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Author Naveed, Muhammad
Mubeen, Samavia
Khan, SamiUllah
Ahmed, Iftikhar
Khalid, Nauman
Suleria, Hafiz Ansar Rasul
Bano, Asghari
Mumtaz, Abdul Samad
Title Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing
Journal name Brazilian Journal of Microbiology   Check publisher's open access policy
ISSN 1517-8382
Publication date 2014-07
Year available 2014
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 45
Issue 3
Start page 985
End page 993
Total pages 9
Place of publication Rio de Janeiro, RJ Brazil
Publisher Sociedade Brasileira de Microbiologia
Collection year 2015
Language eng
Formatted abstract
In the present study, samples of rhizosphere and root nodules were collected from different areas of Pakistan to isolate plant growth promoting rhizobacteria. Identification of bacterial isolates was made by 16S rRNA gene sequence analysis and taxonomical confirmation on EzTaxon Server. The identified bacterial strains were belonged to 5 genera i.e. Ensifer, Bacillus, Pseudomona, Leclercia and Rhizobium. Phylogenetic analysis inferred from 16S rRNA gene sequences showed the evolutionary relationship of bacterial strains with the respective genera. Based on phylogenetic analysis, some candidate novel species were also identified. The bacterial strains were also characterized for morphological, physiological, biochemical tests and glucose dehydrogenase (gdh) gene that involved in the phosphate solublization using cofactor pyrroloquinolone quinone (PQQ). Seven rhizoshperic and 3 root nodulating stains are positive for gdh gene. Furthermore, this study confirms a novel association between microbes and their hosts like field grown crops, leguminous and non-leguminous plants. It was concluded that a diverse group of bacterial population exist in the rhizosphere and root nodules that might be useful in evaluating the mechanisms behind plant microbial interactions and strains QAU-63 and QAU-68 have sequence similarity of 97 and 95% which might be declared as novel after further taxonomic characterization.
Keyword Rhizosphere
Root nodules
Glucose dehydrogenase
Pyrroloquinolone quinone
Plant growth
Growth Promoting Rhizobacteria
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2015 Collection
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