Collecting duct-derived cells display mesenchymal stem cell properties and retain selective in vitro and in vivo epithelial capacity

Li, Joan, Ariunbold, Usukhbayar, Suhaimi, Norseha, Sunn, Nana, Guo, Jinjin, McMahon, Jill A., McMahon, Andrew P. and Little, Melissa (2015) Collecting duct-derived cells display mesenchymal stem cell properties and retain selective in vitro and in vivo epithelial capacity. Journal of the American Society of Nephrology, 26 1: 81-94. doi:10.1681/ASN.2013050517


Author Li, Joan
Ariunbold, Usukhbayar
Suhaimi, Norseha
Sunn, Nana
Guo, Jinjin
McMahon, Jill A.
McMahon, Andrew P.
Little, Melissa
Title Collecting duct-derived cells display mesenchymal stem cell properties and retain selective in vitro and in vivo epithelial capacity
Formatted title
Collecting duct-derived cells display mesenchymal stem cell properties and retain selective in vitro and in vivo epithelial capacity
Journal name Journal of the American Society of Nephrology   Check publisher's open access policy
ISSN 1046-6673
1533-3450
Publication date 2015-01
Year available 2014
Sub-type Article (original research)
DOI 10.1681/ASN.2013050517
Open Access Status
Volume 26
Issue 1
Start page 81
End page 94
Total pages 14
Place of publication Washington, DC, United States
Publisher American Society of Nephrology
Collection year 2015
Language eng
Formatted abstract
We previously described a mesenchymal stem cell (MSC)-like population within the adult mouse kidney that displays long-term colony-forming efficiency, clonogenicity, immunosuppression, and panmesodermal potential. Although phenotypically similar to bone marrow (BM)-MSCs, kidney MSC–like cells display a distinct expression profile. FACS sorting from Hoxb7/enhanced green fluorescent protein (GFP) mice identified the collecting duct as a source of kidney MSC–like cells, with these cells undergoing an epithelial-to-mesenchymal transition to form clonogenic, long-term, self-renewing MSC-like cells. Notably, after extensive passage, kidney MSC–like cells selectively integrated into the aquaporin 2–positive medullary collecting duct when microinjected into the kidneys of neonatal mice. No epithelial integration was observed after injection of BM-MSCs. Indeed, kidney MSC–like cells retained a capacity to form epithelial structures in vitro and in vivo, and conditioned media from these cells supported epithelial repair in vitro. To investigate the origin of kidney MSC–like cells, we further examined Hoxb7+ fractions within the kidney across postnatal development, identifying a neonatal interstitial GFPlo (Hoxb7lo) population displaying an expression profile intermediate between epithelium and interstitium. Temporal analyses with Wnt4GCE/+:R26tdTomato/+ mice revealed evidence for the intercalation of a Wnt4-expressing interstitial population into the neonatal collecting duct, suggesting that such intercalation may represent a normal developmental mechanism giving rise to a distinct collecting duct subpopulation. These results extend previous observations of papillary stem cell activity and collecting duct plasticity and imply a role for such cells in collecting duct formation and, possibly, repair.
Keyword Kidney
Mesenchymal stem cells
Collecting ducts
Epithelial-mesenchymalepithelial transition
Steam cell
Marrow stromal cells
Therapy position statement
Renal progenitor cells
Acute kidney injury
Umbilical cord blood
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 5 June 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
UQ Diamantina Institute Publications
 
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