Distribution and function of HCN channels in the apical dendritic tuft of neocortical pyramidal neurons

Harnett, Mark T, Magee, Jeffrey C and Williams, Stephen R (2015) Distribution and function of HCN channels in the apical dendritic tuft of neocortical pyramidal neurons. Journal of Neuroscience, 35 3: 1024-1037. doi:10.1523/JNEUROSCI.2813-14.2015

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Author Harnett, Mark T
Magee, Jeffrey C
Williams, Stephen R
Title Distribution and function of HCN channels in the apical dendritic tuft of neocortical pyramidal neurons
Journal name Journal of Neuroscience   Check publisher's open access policy
ISSN 1529-2401
0270-6474
Publication date 2015-01-21
Year available 2015
Sub-type Article (original research)
DOI 10.1523/JNEUROSCI.2813-14.2015
Open Access Status File (Publisher version)
Volume 35
Issue 3
Start page 1024
End page 1037
Total pages 14
Place of publication Washington, United States
Publisher Society for Neuroscience
Collection year 2016
Formatted abstract
The apical tuft is the most remote area of the dendritic tree of neocortical pyramidal neurons. Despite its distal location, the apical dendritic tuft of layer 5 pyramidal neurons receives substantial excitatory synaptic drive and actively processes corticocortical input during behavior. The properties of the voltage-activated ion channels that regulate synaptic integration in tuft dendrites have, however, not been thoroughly investigated. Here, we use electrophysiological and optical approaches to examine the subcellular distribution and function of hyperpolarization-activated cyclic nucleotide-gated nonselective cation (HCN) channels in rat layer 5B pyramidal neurons. Outside-out patch recordings demonstrated that the amplitude and properties of ensemble HCN channel activity were uniform in patches excised from distal apical dendritic trunk and tuft sites. Simultaneous apical dendritic tuft and trunk whole-cell current-clamp recordings revealed that the pharmacological blockade of HCN channels decreased voltage compartmentalization and enhanced the generation and spread of apical dendritic tuft and trunk regenerative activity. Furthermore, multisite two-photon glutamate uncaging demonstrated that HCN channels control the amplitude and duration of synaptically evoked regenerative activity in the distal apical dendritic tuft. In contrast, at proximal apical dendritic trunk and somatic recording sites, the blockade of HCN channels decreased excitability. Dynamic-clamp experiments revealed that these compartment-specific actions of HCN channels were heavily influenced by the local and distributed impact of the high density of HCN channels in the distal apical dendritic arbor. The properties and subcellular distribution pattern of HCN channels are therefore tuned to regulate the interaction between integration compartments in layer 5B pyramidal neurons.
Keyword Axon
Dendrite
HCN channel
Neocortex
Synaptic integration
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2016 Collection
 
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