Smoking behaviour modifies IL23r-associated disease risk in patients with Crohn's disease

Doecke, James D, Simms, Lisa A, Zhao, Zhen Zhen, Roberts, Rebecca L, Fowler, Elizabeth V, Croft, Anthony, Lin, Angela, Huang, Ning, Whiteman, David C, Florin, Timothy H J, Barclay, Murray L, Merriman, Tony R, Gearry, Richard B, Montgomery, Grant W and Radford-Smith, Graham L (2015) Smoking behaviour modifies IL23r-associated disease risk in patients with Crohn's disease. Journal of Gastroenterology and Hepatology (Australia), 30 2: 299-307. doi:10.1111/jgh.12674


Author Doecke, James D
Simms, Lisa A
Zhao, Zhen Zhen
Roberts, Rebecca L
Fowler, Elizabeth V
Croft, Anthony
Lin, Angela
Huang, Ning
Whiteman, David C
Florin, Timothy H J
Barclay, Murray L
Merriman, Tony R
Gearry, Richard B
Montgomery, Grant W
Radford-Smith, Graham L
Title Smoking behaviour modifies IL23r-associated disease risk in patients with Crohn's disease
Journal name Journal of Gastroenterology and Hepatology (Australia)   Check publisher's open access policy
ISSN 1440-1746
0815-9319
Publication date 2015-02-01
Year available 2015
Sub-type Article (original research)
DOI 10.1111/jgh.12674
Volume 30
Issue 2
Start page 299
End page 307
Total pages 9
Place of publication Richmond, Australia
Publisher Wiley-Blackwell Publishing Asia
Collection year 2016
Language eng
Formatted abstract
Background and Aim
The etiology of Crohn's disease (CD) implicates both genetic and environmental factors. Smoking behavior is one environmental risk factor to play a role in the development of CD. The study aimed to assess the contribution of the interleukin 23 receptor (IL23R) in determining disease susceptibility in two independent cohorts of CD, and to investigate the interactions between IL23R variants, smoking behavior, and CD-associated genes, NOD2 and ATG16L1.

Methods
Ten IL23R single-nucleotide polymorphisms (SNPs) were genotyped in 675 CD cases, and 1255 controls from Brisbane, Australia (dataset 1). Six of these SNPs were genotyped in 318 CD cases and 533 controls from Canterbury, New Zealand (dataset 2). Case–control analysis of genotype and allele frequencies, and haplotype analysis for all SNPs was conducted.

Results
We demonstrate a strong increased CD risk for smokers in both datasets (odds ratio 3.77, 95% confidence interval 2.88–4.94), and an additive interaction between IL23R SNPs and cigarette smoking. Ileal involvement was a consistent marker of strong SNP–CD association (P ≤ 0.001), while the lowest minor allele frequencies for location were found in those with colonic CD (L2). Three haplotype blocks were identified across the 10 IL23R SNPs conferring different risk of CD. Haplotypes conferred no further risk of CD when compared with single SNP analyses.

Conclusion
IL23R gene variants determine CD susceptibility in the Australian and New Zealand population, particularly ileal CD. A strong additive interaction exists between IL23R SNPs and smoking behavior resulting in a dramatic increase in disease risk depending upon specific genetic background.
Keyword ATG16L1
Crohn's disease
IL23R
Interleukin 23 receptor
NOD2
Smoking
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Medicine Publications
 
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