New techniques for biopsy and culture of human olfactory epithelial neurons

Feron, F, Perry, C, McGrath, J and Mackay-Sim, A (1998) New techniques for biopsy and culture of human olfactory epithelial neurons. JAMA Otolaryngology. Head & Neck Surgery, 124 8: 861-866. doi:10.1001/archotol.124.8.861

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ35037_OA.pdf Full text (open access) application/pdf 452.23KB 0

Author Feron, F
Perry, C
McGrath, J
Mackay-Sim, A
Title New techniques for biopsy and culture of human olfactory epithelial neurons
Journal name JAMA Otolaryngology. Head & Neck Surgery   Check publisher's open access policy
ISSN 2168-6181
2168-619X
Publication date 1998
Sub-type Article (original research)
DOI 10.1001/archotol.124.8.861
Open Access Status File (Publisher version)
Volume 124
Issue 8
Start page 861
End page 866
Total pages 6
Place of publication Chicago, IL, United States
Publisher American Medical Association
Language eng
Abstract Objective: To improve the success of culturing olfactory neurons from human nasal mucosa by investigating the intranasal distribution of the olfactory epithelium and devising new techniques for growing human olfactory epithelium in vitro. Design: Ninety-seven biopsy specimens were obtained from 33 individuals, aged 21 to 74 years, collected from 6 regions of the nasal cavity. Each biopsy specimen was bisected, and 1 piece was processed for immunohistochemistry or electron microscopy while the other piece was dissected further for explant culture. Four culture techniques were performed, including whole explants and explanted biopsy slices. Five days after plating, neuronal differentiation was induced by means of a medium that contained basic fibroblast growth factor. After another 5 days, cultures were processed for immunocytochemical analysis. Results: The probability of finding olfactory epithelium in a biopsy specimen ranged from 30% to 76%, depending on its location. The dorsoposterior regions of the nasal septum and the superior turbinate provided the highest probability, but, surprisingly, olfactory epithelium was also found anteriorly and ventrally on both septum and turbinates. A new method of culturing the olfactory epithelium was devised. This slice culture technique improved the success rate for generating olfactory neurons from 10% to 90%. Conclusions: This study explains and overcomes most of the variability in the success in observing neurogenesis in cultures of adult human olfactory epithelium. The techniques presented here make the human olfactory epithelium a useful model for clinical research into certain olfactory dysfunctions and a model for the causes of neurodevelopmental and neurodegenerative diseases.
Keyword Otorhinolaryngology
Surgery
Adult
Cell
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 157 times in Thomson Reuters Web of Science Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 13 Aug 2007, 10:34:16 EST