Preparation, characterization, in vitro drug release and biological studies of curcumin loaded dextran sulphate–chitosan nanoparticles

Anitha, A., Deepagan, V. G., Divya Rani, V. V., Nair, S. V. and Jayakumar, R. (2011) Preparation, characterization, in vitro drug release and biological studies of curcumin loaded dextran sulphate–chitosan nanoparticles. Carbohydrate Polymers, 84 3: 1158-1164. doi:10.1016/j.carbpol.2011.01.005

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Author Anitha, A.
Deepagan, V. G.
Divya Rani, V. V.
Nair, S. V.
Jayakumar, R.
Title Preparation, characterization, in vitro drug release and biological studies of curcumin loaded dextran sulphate–chitosan nanoparticles
Formatted title
Preparation, characterization, in vitro drug release and biological studies of curcumin loaded dextran sulphate–chitosan nanoparticles
Journal name Carbohydrate Polymers   Check publisher's open access policy
ISSN 0144-8617
1879-1344
Publication date 2011-03-17
Sub-type Article (original research)
DOI 10.1016/j.carbpol.2011.01.005
Volume 84
Issue 3
Start page 1158
End page 1164
Total pages 7
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Language eng
Formatted abstract
Nanoformulation of curcumin, (a low molecular weight hydrophobic drug) was prepared by using dextran sulphate and chitosan. The developed nanoparticles were characterized by Dynamic Light Scattering measurements (DLS), Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD) and Differential Thermal Analysis (DTA). The prepared system showed an average size of 200-220 nm with a zeta potential value of -30 mV and showed ∼74% drug entrapment efficiency. In vitro drug release studies showed a controlled and pH dependent curcumin release over a period of one week. The cytocompatibility of bare nanoparticles was verified by MTT assay; cellular internalisation of curcumin loaded nanoparticles was confirmed by fluorescent imaging and quantified spectrophotometrically, anticancer activity of curcumin loaded nanoparticles was proved by MTT assay and reconfirmed by apoptosis assay (FACS). The results showed preferential killing of cancer cells compared to normal cells by the curcumin-loaded nanoparticles. Thus the developed curcumin loaded nanoformulation could be a promising candidate in cancer therapy.
Keyword Chitosan
Dextran sulfate
Nanoparticles
Curcumin
Controlled delivery
Cytotoxicity
Cellular uptake studies
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Wed, 28 Jan 2015, 02:24:48 EST by Anitha Sudheesh Kumar on behalf of School of Chemistry & Molecular Biosciences