Cytorhabdovirus phosphoprotein shows RNA silencing suppressor activity in plants, but not in insect cells

Mann, Krin S., Johnson, Karyn N. and Dietzgen, Ralf G. (2015) Cytorhabdovirus phosphoprotein shows RNA silencing suppressor activity in plants, but not in insect cells. Virology, 476 413-418. doi:10.1016/j.virol.2014.12.023


Author Mann, Krin S.
Johnson, Karyn N.
Dietzgen, Ralf G.
Title Cytorhabdovirus phosphoprotein shows RNA silencing suppressor activity in plants, but not in insect cells
Journal name Virology   Check publisher's open access policy
ISSN 1096-0341
0042-6822
Publication date 2015-02-01
Year available 2015
Sub-type Letter to editor, brief commentary or brief communication
DOI 10.1016/j.virol.2014.12.023
Volume 476
Start page 413
End page 418
Total pages 6
Place of publication Waltham, MA United States
Publisher Academic Press
Collection year 2016
Language eng
Formatted abstract
RNA silencing in plants and insects provides an antiviral defense and as a countermeasure most viruses encode RNA silencing suppressors (RSS). For the family Rhabdoviridae, no detailed functional RSS studies have been reported in plant hosts and insect vectors. In agroinfiltrated Nicotiana benthamiana leaves we show for the first time for a cytorhabdovirus, lettuce necrotic yellows virus (LNYV), that one of the nucleocapsid core proteins, phosphoprotein (P) has relatively weak local RSS activity and delays systemic silencing of a GFP reporter. Analysis of GFP small RNAs indicated that the P protein did not prevent siRNA accumulation. To explore RSS activity in insects, we used a Flock House virus replicon system in Drosophila S2 cells. In contrast to the plant host, LNYV P protein did not exhibit RSS activity in the insect cells. Taken together our results suggest that P protein may target plant-specific components of RNA silencing post siRNA biogenesis.
Keyword Lettuce necrotic yellows virus
Green fluorescent protein
RNA silencing suppression
Agroinfiltration
Viral protein expression
Drosophila S2 cells
Flock House virus
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Letter to editor, brief commentary or brief communication
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2016 Collection
School of Biological Sciences Publications
 
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