Late passage human fibroblasts induced to pluripotency are capable of directed neuronal differentiation

Liu, Jun, Sumer, Huseyin, Leung, Jessie, Upton, Kyle, Dottori, Mirella, Pebay, Alice and Verma, Paul J. (2011) Late passage human fibroblasts induced to pluripotency are capable of directed neuronal differentiation. Cell Transplantation, 20 2: 193-203. doi:10.3727/096368910X514305

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Author Liu, Jun
Sumer, Huseyin
Leung, Jessie
Upton, Kyle
Dottori, Mirella
Pebay, Alice
Verma, Paul J.
Title Late passage human fibroblasts induced to pluripotency are capable of directed neuronal differentiation
Journal name Cell Transplantation   Check publisher's open access policy
ISSN 0963-6897
1555-3892
Publication date 2011-02
Year available 2011
Sub-type Article (original research)
DOI 10.3727/096368910X514305
Open Access Status File (Author Post-print)
Volume 20
Issue 2
Start page 193
End page 203
Total pages 11
Place of publication Putnam Valley, United States
Publisher Cognizant Communication Corporation
Language eng
Abstract It is possible to generate induced pluripotent stem (iPS) cells from mouse and human somatic cells by ectopic expression of defined sets of transcription factors. However, the recommendation that somatic cells should be utilized at early passages for induced reprogramming limits their therapeutic application. Here we report successful reprogramming of human fibroblasts after more than 20 passages in vitro, to a pluripotent state with four transcription factors: Oct4, Sox2, Klf4, and c-Myc. The late passage-derived human iPS cells resemble human embryonic stem cells in morphology, cell surface antigens, pluripotent gene expression profiles, and epigenetic states. Moreover, these iPS cells differentiate into cell types representative of the three germ layers in teratomas in vivo, and directed neuronal differentiation in vitro.
Keyword Human fibroblasts
Late passage
Neuronal differentiation
Pluripotent reprogramming
Stem cell
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Mater Research Institute-UQ (MRI-UQ)
 
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