Myogenesis in small and large ovine fetuses at three stages of pregnancy

Quigley, S. P., Greenwood, P. L., Kleemann, D. O., Owens, J. A., Bawden, C. S. and Nattrass, G. S. (2015) Myogenesis in small and large ovine fetuses at three stages of pregnancy. Animal Production Science, 55 2: 207-212. doi:10.1071/AN14203

Author Quigley, S. P.
Greenwood, P. L.
Kleemann, D. O.
Owens, J. A.
Bawden, C. S.
Nattrass, G. S.
Title Myogenesis in small and large ovine fetuses at three stages of pregnancy
Journal name Animal Production Science   Check publisher's open access policy
ISSN 1836-5787
Publication date 2015
Year available 2014
Sub-type Article (original research)
DOI 10.1071/AN14203
Open Access Status
Volume 55
Issue 2
Start page 207
End page 212
Total pages 6
Place of publication Clayton, VIC Australia
Publisher CSIRO Publishing
Collection year 2015
Language eng
Formatted abstract
Perturbations of the prenatal environment may influence fetal muscle development. This study investigated muscle cellularity and mRNA abundance of myogenic genes in fetal sheep divergent in their patterns of growth. Muscle samples were obtained from small and large fetuses on Days 50, 92 and 133 of pregnancy. Number of myofibres in the semitendinosus muscle increased between Day 92 and 133 of pregnancy, but did not differ between small and large fetuses at either stage of pregnancy. The semitendinosus of small fetuses had smaller cross-sectional areas of myofibres than did those of their large counterparts on Day 133 of pregnancy. The semitendinosus of small fetuses also had lower DNA concentration on Day 92 and lower protein concentration on Day 133 than did those of large fetuses. The mRNA levels of the myogenic regulatory factors (MRFs), myostatin, the insulin-like growth factors and embryonic myosin in fetal muscles varied with the stage of development, but no differences occurred in response to divergent fetal growth. Myostatin mRNA was more abundant in the semitendinosus than in the supraspinatus muscle on Days 92 and 133, as were myogenic regulatory factors, myf-5, myf-6 and follistatin mRNA on Day 133. The results indicated that muscle growth but not the number of myofibres in fetal sheep is modified by restricted fetal growth, and that genes that regulate muscle development are affected by the stage of development in an anatomical muscle-specific manner.
Keyword Gene expression
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 19 Dec 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2015 Collection
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Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
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