The affinity of magnetic microspheres for Schistosoma eggs

Candido, Renata R. F., Favero, Vivian, Duke, Mary, Karl, Stephan, Gutierrez, Lucia, Woodward, Robert C., Graeff-Teixeira, Carlos, Jones, Malcom K. and St. Pierre, Timothy G. (2015) The affinity of magnetic microspheres for Schistosoma eggs. International Journal for Parasitology, 45 1: 43-50. doi:10.1016/j.ijpara.2014.08.011


Author Candido, Renata R. F.
Favero, Vivian
Duke, Mary
Karl, Stephan
Gutierrez, Lucia
Woodward, Robert C.
Graeff-Teixeira, Carlos
Jones, Malcom K.
St. Pierre, Timothy G.
Title The affinity of magnetic microspheres for Schistosoma eggs
Formatted title
The affinity of magnetic microspheres for Schistosoma eggs
Journal name International Journal for Parasitology   Check publisher's open access policy
ISSN 1879-0135
0020-7519
Publication date 2015-01
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.ijpara.2014.08.011
Open Access Status
Volume 45
Issue 1
Start page 43
End page 50
Total pages 8
Place of publication Oxford, United Kingdom
Publisher Elsevier
Collection year 2014
Language eng
Formatted abstract
Schistosomiasis is a chronic parasitic disease of humans, with two species primarily causing the intestinal infection: Schistosoma mansoni and Schistosoma japonicum. Traditionally, diagnosis of schistosomiasis is achieved through direct visualisation of eggs in faeces using techniques that lack the sensitivity required to detect all infections, especially in areas of low endemicity. A recently developed method termed Helmintex™ is a very sensitive technique for detection of Schistosoma eggs and exhibits 100% sensitivity at 1.3 eggs per gram of faeces, enough to detect even low-level infections. The Helminthex™ method is based on the interaction of magnetic microspheres and schistosome eggs. Further understanding the underlying egg-microsphere interactions would enable a targeted optimisation of egg-particle binding and may thus enable a significant improvement of the Helmintex™ method and diagnostic sensitivity in areas with low infection rates. We investigated the magnetic properties of S. mansoni and S. japonicum eggs and their interactions with microspheres with different magnetic properties and surface functionalization. Eggs of both species exhibited higher binding affinity to the magnetic microspheres than the non-magnetic microspheres. Binding efficiency was further enhanced if the particles were coated with streptavidin. Schistosoma japonicum eggs bound more microspheres compared with S. mansoni. However, distinct differences within eggs of each species were also observed when the distribution of the number of microspheres bound per egg was modelled with double Poisson distributions. Using this approach, both S. japonicum and S. mansoni eggs fell into two groups, one having greater affinity for magnetic microspheres than the other, indicating that not all eggs of a species exhibit the same binding affinity. Our observations suggest that interaction between the microspheres and eggs is more likely to be related to surface charge-based electrostatic interactions between eggs and magnetic iron oxide rather than through a direct magnetic interaction.
Keyword Schistosomiasis
Diagnosis
Low endemicity
Helmintex™
Magnetic properties
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 7 Oct 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Veterinary Science Publications
 
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